BACKGROUND: Confocal laser enables in vivo real-time histopathology of the mucosa layer of gastrointestinal tract. The aim of this study was to assess the feasibility and the role of probe-based confocal laser endomicroscopy in extemporary examination of staple rings of patients with colorectal cancer. METHODS: This was a prospective, single-center pilot study. Patients who underwent end-to-end stapled surgical resection for colorectal cancer were included. Confocal imaging was analyzed with great attention to image quality evaluation of cellular morphology and cellular structures of the serosal, muscular, and mucosal layers of the doughnuts than comparing results with definitive histopathology. RESULTS: Twenty-six doughnuts were analyzed. Real-time video sequences were obtained in all patients, with a total of 204 mosaic images. For each doughnut, most of the images were adequate for evaluation of cellular morphology and cellular structure of the serosal, muscular, and mucosal layers. CONCLUSIONS: Perioperative assessment of doughnut tissues in patients with colorectal cancer by confocal laser endomicroscopy is feasible and safe. Prospective studies are warranted for further evaluation of the clinical impact of this technology during surgery.
BACKGROUND: Confocal laser enables in vivo real-time histopathology of the mucosa layer of gastrointestinal tract. The aim of this study was to assess the feasibility and the role of probe-based confocal laser endomicroscopy in extemporary examination of staple rings of patients with colorectal cancer. METHODS: This was a prospective, single-center pilot study. Patients who underwent end-to-end stapled surgical resection for colorectal cancer were included. Confocal imaging was analyzed with great attention to image quality evaluation of cellular morphology and cellular structures of the serosal, muscular, and mucosal layers of the doughnuts than comparing results with definitive histopathology. RESULTS: Twenty-six doughnuts were analyzed. Real-time video sequences were obtained in all patients, with a total of 204 mosaic images. For each doughnut, most of the images were adequate for evaluation of cellular morphology and cellular structure of the serosal, muscular, and mucosal layers. CONCLUSIONS: Perioperative assessment of doughnut tissues in patients with colorectal cancer by confocal laser endomicroscopy is feasible and safe. Prospective studies are warranted for further evaluation of the clinical impact of this technology during surgery.
Authors: Valentin Becker; Michael B Wallace; Paul Fockens; Stefan von Delius; Timothy A Woodward; Massimo Raimondo; Rogier P Voermans; Alexander Meining Journal: Gastrointest Endosc Date: 2010-04-24 Impact factor: 9.427
Authors: Antonio Rispo; Fabiana Castiglione; Stefania Staibano; Dario Esposito; Francesco Maione; Maria Siano; Francesca Salvatori; Stefania Masone; Marcello Persico; Giovanni Domenico De Palma Journal: World J Gastrointest Endosc Date: 2012-09-16
Authors: Bun Kim; Yon Hee Kim; Soo Jung Park; Jae Hee Cheon; Tae Il Kim; Won Ho Kim; Hoguen Kim; Sung Pil Hong Journal: Surg Endosc Date: 2016-06-20 Impact factor: 4.584
Authors: James R Holden; Pam McIntosh; Garrett G R J Johnson; Jason Park; David J Hochman; Ashley Vergis; Benson Yip; Ramzi M Helewa; Eric Hyun Journal: BJS Open Date: 2022-09-02