Literature DB >> 2039210

Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus.

M Ligozzi1, G M Rossolini, E A Tonin, R Fontana.   

Abstract

A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.

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Year:  1991        PMID： 2039210      PMCID： PMC245053          DOI： 10.1128/AAC.35.3.575

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  19 in total

1.  Inactivation of benzylpenicillin and methicillin by hospital staphylococci.

Authors:  G A AYLIFFE; M BARBER
Journal:  Br Med J       Date:  1963-07-27

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Regulation of penicillin-binding protein activity: description of a methicillin-inducible penicillin-binding protein in Staphylococcus aureus.

Authors:  L Rossi; E Tonin; Y R Cheng; R Fontana
Journal:  Antimicrob Agents Chemother       Date:  1985-05       Impact factor: 5.191

4.  Susceptibility of the "penicillinase-resistant" penicillins and cephalosporins to penicillinase of Staphylococcus aureus.

Authors:  R W Lacey; A Stokes
Journal:  J Clin Pathol       Date:  1977-01       Impact factor: 3.411

5.  Intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus.

Authors:  D F Brown; P E Reynolds
Journal:  FEBS Lett       Date:  1980-12-29       Impact factor: 4.124

6.  Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus.

Authors:  B J Hartman; A Tomasz
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

7.  The role of beta-lactamase in staphylococcal resistance to penicillinase-resistant penicillins and cephalosporins.

Authors:  L K McDougal; C Thornsberry
Journal:  J Clin Microbiol       Date:  1986-05       Impact factor: 5.948

8.  Beta-lactam-specific resistant mutants of Staphylococcus aureus.

Authors:  E Tonin; A Tomasz
Journal:  Antimicrob Agents Chemother       Date:  1986-10       Impact factor: 5.191

9.  Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus.

Authors:  M Matsuhashi; M D Song; F Ishino; M Wachi; M Doi; M Inoue; K Ubukata; N Yamashita; M Konno
Journal:  J Bacteriol       Date:  1986-09       Impact factor: 3.490

10.  Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.

Authors:  C H O'Callaghan; A Morris; S M Kirby; A H Shingler
Journal:  Antimicrob Agents Chemother       Date:  1972-04       Impact factor: 5.191
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  14 in total

Review 1.  Molecular detection of antimicrobial resistance.

Authors:  A C Fluit; M R Visser; F J Schmitz
Journal:  Clin Microbiol Rev       Date:  2001-10       Impact factor: 26.132

2.  Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction.

Authors:  S C Predari; M Ligozzi; R Fontana
Journal:  Antimicrob Agents Chemother       Date:  1991-12       Impact factor: 5.191

3.  Comparison of disk diffusion, the E test, and detection of mecA for determination of methicillin resistance in coagulase-negative staphylococci.

Authors:  J G Mulder
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-07       Impact factor: 3.267

4.  Comparison of phenotypic methods and DNA hybridization for detection of methicillin-resistant Staphylococcus aureus.

Authors:  P Richard; M Meyran; E Carpentier; A Thabaut; H B Drugeon
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

5.  Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products.

Authors:  K Ubukata; S Nakagami; A Nitta; A Yamane; S Kawakami; M Sugiura; M Konno
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

6.  Detection of methicillin-resistant staphylococci by using the polymerase chain reaction.

Authors:  S Unal; J Hoskins; J E Flokowitsch; C Y Wu; D A Preston; P L Skatrud
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

7.  Detection of the Staphylococcal mecA gene by chemiluminescent DNA hybridization.

Authors:  C P Kolbert; J E Connolly; M J Lee; D H Persing
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948

8.  Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory.

Authors:  D J Geha; J R Uhl; C A Gustaferro; D H Persing
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

9.  Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphylococcus aureus.

Authors:  M Shimaoka; M Yoh; A Segawa; Y Takarada; K Yamamoto; T Honda
Journal:  J Clin Microbiol       Date:  1994-08       Impact factor: 5.948

10.  Revised interpretation of oxacillin MICs for Staphylococcus epidermidis based on mecA detection.

Authors:  C L McDonald; W E Maher; R J Fass
Journal:  Antimicrob Agents Chemother       Date:  1995-04       Impact factor: 5.191
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