Paenibacillus rigui sp. nov., isolated from a freshwater wetland.

A rod-shaped, endospore-forming, Gram-reaction-variable bacterial strain, designated WPCB173(T), was isolated from freshwater collected from the Woopo wetland, Republic of Korea. Based on its phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analysis, the isolate was identified as being a member of the genus Paenibacillus. Major polar lipids present in strain WPCB173(T) included phosphatidylethanolamine and several unidentified phospholipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acid was anteiso-C₁₅:₀ (65.2  %). The DNA G+C content was 48.3 %. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WPCB173(T) clustered with species of the genus Paenibacillus, its closest neighbours being Paenibacillus chinjuensis WN9(T) (96.7  %) and Paenibacillus soli DCY03(T) (96.4  %). DNA-DNA hybridization of strain WPCB173(T) with P. soli DCY03(T) and P. chinjuensis WN9(T) showed relatedness values of only 10 and 19  %, respectively. On the basis of the phenotypic and phylogenetic evidence, strain WPCB173(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus rigui sp. nov. is proposed. The type strain of the novel species is WPCB173(T) (=KCTC 13282(T) =JCM 16352(T)).