| Literature DB >> 20368420 |
Miao He1, Mohammed Sebaihia, Trevor D Lawley, Richard A Stabler, Lisa F Dawson, Melissa J Martin, Kathryn E Holt, Helena M B Seth-Smith, Michael A Quail, Richard Rance, Karen Brooks, Carol Churcher, David Harris, Stephen D Bentley, Christine Burrows, Louise Clark, Craig Corton, Vicky Murray, Graham Rose, Scott Thurston, Andries van Tonder, Danielle Walker, Brendan W Wren, Gordon Dougan, Julian Parkhill.
Abstract
Clostridium difficile has rapidly emerged as the leading cause of antibiotic-associated diarrheal disease, with the transcontinental spread of various PCR ribotypes, including 001, 017, 027 and 078. However, the genetic basis for the emergence of C. difficile as a human pathogen is unclear. Whole genome sequencing was used to analyze genetic variation and virulence of a diverse collection of thirty C. difficile isolates, to determine both macro and microevolution of the species. Horizontal gene transfer and large-scale recombination of core genes has shaped the C. difficile genome over both short and long time scales. Phylogenetic analysis demonstrates C. difficile is a genetically diverse species, which has evolved within the last 1.1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages.Entities:
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Year: 2010 PMID： 20368420 PMCID： PMC2867753 DOI： 10.1073/pnas.0914322107
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205