Literature DB >> 20363899

Single-step affinity purification for fungal proteomics.

Hui-Lin Liu1, Aysha H Osmani, Leena Ukil, Sunghun Son, Sarine Markossian, Kuo-Fang Shen, Meera Govindaraghavan, Archana Varadaraj, Shahr B Hashmi, Colin P De Souza, Stephen A Osmani.   

Abstract

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.

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Year:  2010        PMID： 20363899      PMCID： PMC2863959          DOI： 10.1128/EC.00032-10

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  10 in total

1.  Degradation of ribonuclease by subtilisin.

Authors:  S M KALMAN; K LINDERSTRØM-LANG; M OTTESEN; F M RICHARDS
Journal:  Biochim Biophys Acta       Date:  1955-02

2.  On an active intermediate produced during the digestion of ribonuclease by subtilisin.

Authors:  F M RICHARDS
Journal:  C R Trav Lab Carlsberg Chim       Date:  1955

3.  Titration of amino groups released during the digestion of ribonuclease by subtilisin.

Authors:  F M RICHARDS
Journal:  C R Trav Lab Carlsberg Chim       Date:  1955

4.  Copy number suppressors of the Aspergillus nidulans nimA1 mitotic kinase display distinctive and highly dynamic cell cycle-regulated locations.

Authors:  Leena Ukil; Archana Varadaraj; Meera Govindaraghavan; Hui-Lin Liu; Stephen A Osmani
Journal:  Eukaryot Cell       Date:  2008-10-17

5.  Systematic deletion and mitotic localization of the nuclear pore complex proteins of Aspergillus nidulans.

Authors:  Aysha H Osmani; Jonathan Davies; Hui-Lin Liu; Aaron Nile; Stephen A Osmani
Journal:  Mol Biol Cell       Date:  2006-09-20       Impact factor: 4.138

6.  A versatile and efficient gene-targeting system for Aspergillus nidulans.

Authors:  Tania Nayak; Edyta Szewczyk; C Elizabeth Oakley; Aysha Osmani; Leena Ukil; Sandra L Murray; Michael J Hynes; Stephen A Osmani; Berl R Oakley
Journal:  Genetics       Date:  2005-12-30       Impact factor: 4.562

7.  Analysis of all protein phosphatase genes in Aspergillus nidulans identifies a new mitotic regulator, fcp1.

Authors:  Sunghun Son; Stephen A Osmani
Journal:  Eukaryot Cell       Date:  2009-01-30

8.  Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans.

Authors:  Lin Yang; Leena Ukil; Aysha Osmani; Francis Nahm; Jonathan Davies; Colin P C De Souza; Xiaowei Dou; Ariadna Perez-Balaguer; Stephen A Osmani
Journal:  Eukaryot Cell       Date:  2004-10

9.  Ribonuclease S-peptide as a carrier in fusion proteins.

Authors:  J S Kim; R T Raines
Journal:  Protein Sci       Date:  1993-03       Impact factor: 6.725

10.  The three fungal transmembrane nuclear pore complex proteins of Aspergillus nidulans are dispensable in the presence of an intact An-Nup84-120 complex.

Authors:  Hui-Lin Liu; Colin P C De Souza; Aysha H Osmani; Stephen A Osmani
Journal:  Mol Biol Cell       Date:  2008-11-19       Impact factor: 4.138
  10 in total
  30 in total

1.  The Polo-like kinase PLKA in Aspergillus nidulans is not essential but plays important roles during vegetative growth and development.

Authors:  Klarita Mogilevsky; Amandeep Glory; Catherine Bachewich
Journal:  Eukaryot Cell       Date:  2011-12-02

2.  Insights into dynamic mitotic chromatin organization through the NIMA kinase suppressor SonC, a chromatin-associated protein involved in the DNA damage response.

Authors:  Jennifer R Larson; Eric M Facemyer; Kuo-Fang Shen; Leena Ukil; Stephen A Osmani
Journal:  Genetics       Date:  2013-11-08       Impact factor: 4.562

3.  The SrkA Kinase Is Part of the SakA Mitogen-Activated Protein Kinase Interactome and Regulates Stress Responses and Development in Aspergillus nidulans.

Authors:  Rafael Jaimes-Arroyo; Fernando Lara-Rojas; Özgür Bayram; Oliver Valerius; Gerhard H Braus; Jesús Aguirre
Journal:  Eukaryot Cell       Date:  2015-03-27

4.  Mitochondrial Citrate Transporters CtpA and YhmA Are Required for Extracellular Citric Acid Accumulation and Contribute to Cytosolic Acetyl Coenzyme A Generation in Aspergillus luchuensis mut. kawachii.

Authors:  Chihiro Kadooka; Kosuke Izumitsu; Masahira Onoue; Kayu Okutsu; Yumiko Yoshizaki; Kazunori Takamine; Masatoshi Goto; Hisanori Tamaki; Taiki Futagami
Journal:  Appl Environ Microbiol       Date:  2019-04-04       Impact factor: 4.792

5.  TRAPPII regulates exocytic Golgi exit by mediating nucleotide exchange on the Ypt31 ortholog RabERAB11.

Authors:  Mario Pinar; Herbert N Arst; Areti Pantazopoulou; Víctor G Tagua; Vivian de los Ríos; Javier Rodríguez-Salarichs; J Fernando Díaz; Miguel A Peñalva
Journal:  Proc Natl Acad Sci U S A       Date:  2015-03-23       Impact factor: 11.205

6.  SUMOlock reveals a more complete Aspergillus nidulans SUMOylome.

Authors:  Tetsuya Horio; Edyta Szewczyk; C Elizabeth Oakley; Aysha H Osmani; Stephen A Osmani; Berl R Oakley
Journal:  Fungal Genet Biol       Date:  2019-03-05       Impact factor: 3.495

7.  veA-dependent RNA-pol II transcription elongation factor-like protein, RtfA, is associated with secondary metabolism and morphological development in Aspergillus nidulans.

Authors:  Vellaisamy Ramamoorthy; Sourabha Shantappa; Sourabh Dhingra; Ana M Calvo
Journal:  Mol Microbiol       Date:  2012-07-12       Impact factor: 3.501

8.  Restraint of the G2/M transition by the SR/RRM family mRNA shuttling binding protein SNXAHRB1 in Aspergillus nidulans.

Authors:  Steven W James; Travis Banta; James Barra; Lorela Ciraku; Clifford Coile; Zach Cuda; Ryan Day; Cheshil Dixit; Steven Eastlack; Anh Giang; James Goode; Alexis Guice; Yulon Huff; Sara Humbert; Christina Kelliher; Julie Kobie; Emily Kohlbrenner; Faustin Mwambutsa; Amanda Orzechowski; Kristin Shingler; Casey Spell; Sarah Lea Anglin
Journal:  Genetics       Date:  2014-08-07       Impact factor: 4.562

Review 9.  Microtubule-based transport in filamentous fungi.

Authors:  Martin J Egan; Mark A McClintock; Samara L Reck-Peterson
Journal:  Curr Opin Microbiol       Date:  2012-11-02       Impact factor: 7.934

10.  The Set1/COMPASS histone H3 methyltransferase helps regulate mitosis with the CDK1 and NIMA mitotic kinases in Aspergillus nidulans.

Authors:  Meera Govindaraghavan; Sarah Lea Anglin; Aysha H Osmani; Stephen A Osmani
Journal:  Genetics       Date:  2014-05-15       Impact factor: 4.562
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