Literature DB >> 20347168

Relative biological effectiveness of pulsed and continuous 20 MeV protons for micronucleus induction in 3D human reconstructed skin tissue.

Thomas E Schmid1, Günther Dollinger, Volker Hable, Christoph Greubel, Olga Zlobinskaya, Dörte Michalski, Michael Molls, Barbara Röper.   

Abstract

BACKGROUND AND
PURPOSE: Laser accelerated radiotherapy is a prospect for cancer treatment with proton and/or carbon ion beams that is currently under fast development. In principal, ultra fast, high-energy laser pulses will lead to a "pulsed" delivery of the induced ion beam with pulse durations of 1ns and below, whereas conventional proton beams deriving from a cyclotron or synchrotron apply the dose within 100 ms ("continuous").
MATERIALS AND METHODS: A simulation of both irradiation modes could be established at the Munich tandem accelerator with a 20MeV proton beam, and a wide-field fast scanning system was implemented that allowed for application of up to 5 Gy per tissue voxel in a single pulse. The relative biological effectiveness (RBE) of pulsed and continuous modes of irradiation with 20 MeV protons relative to the reference radiation 70 kV X-rays was examined in a human tissue model (3D human reconstructed skin, EpiDermFT) which preserves the three-dimensional geometric arrangement and communication of cells present in tissues in vivo. Using the induction of micronuclei (MN) in keratinocytes as the biological endpoint, the RBE was calculated as the ratio between the dose of 70 kV X-rays and 3 Gy of 20 MeV protons (pulsed or continuous) which produced equal response.
RESULTS: For pulsed and continuous 20 MV proton exposures of the human skin model, RBE values of 1.08+/-0.20 and 1.22+/-0.15 versus 70 kV X-rays were obtained in a first experiment and 1.00+/-0.14 and 1.13+/-0.14 in a second experiment during distinct beam access times, respectively. The approximately 10% difference in RBE between the respective irradiation modes in both experiments was associated with large uncertainties which were not statistically significant (p approximately 0.5).
CONCLUSION: These findings represent an important step on the way towards application of laser-accelerated protons for clinical radiotherapy. Further clinically relevant endpoints in normal and tumor tissue have to be evaluated. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

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Year:  2010        PMID: 20347168     DOI: 10.1016/j.radonc.2010.03.010

Source DB:  PubMed          Journal:  Radiother Oncol        ISSN: 0167-8140            Impact factor:   6.280


  22 in total

1.  Induction and repair of DNA double-strand breaks assessed by gamma-H2AX foci after irradiation with pulsed or continuous proton beams.

Authors:  O Zlobinskaya; G Dollinger; D Michalski; V Hable; C Greubel; G Du; G Multhoff; B Röper; M Molls; T E Schmid
Journal:  Radiat Environ Biophys       Date:  2012-01-07       Impact factor: 1.925

Review 2.  Microirradiation techniques in radiobiological research.

Authors:  Guido A Drexler; Miguel J Ruiz-Gómez
Journal:  J Biosci       Date:  2015-09       Impact factor: 1.826

3.  Scanning irradiation device for mice in vivo with pulsed and continuous proton beams.

Authors:  Christoph Greubel; Walter Assmann; Christian Burgdorf; Günther Dollinger; Guanghua Du; Volker Hable; Alexander Hapfelmeier; Ralf Hertenberger; Peter Kneschaurek; Dörte Michalski; Michael Molls; Sabine Reinhardt; Barbara Röper; Stefan Schell; Thomas E Schmid; Christian Siebenwirth; Tatiana Wenzl; Olga Zlobinskaya; Jan J Wilkens
Journal:  Radiat Environ Biophys       Date:  2011-05-10       Impact factor: 1.925

4.  New challenges in radiobiology research with microbeams.

Authors:  Marco Durante; Anna A Friedl
Journal:  Radiat Environ Biophys       Date:  2011-06-12       Impact factor: 1.925

5.  The influence of the channel size on the reduction of side effects in microchannel proton therapy.

Authors:  Stefanie Girst; Christoph Greubel; Judith Reindl; Christian Siebenwirth; Olga Zlobinskaya; Günther Dollinger; Thomas E Schmid
Journal:  Radiat Environ Biophys       Date:  2015-05-09       Impact factor: 1.925

Review 6.  Spatially fractionated proton minibeams.

Authors:  Juergen Meyer; John Eley; Thomas E Schmid; Stephanie E Combs; Remi Dendale; Yolanda Prezado
Journal:  Br J Radiol       Date:  2018-11-07       Impact factor: 3.039

7.  Reduced side effects by proton microchannel radiotherapy: study in a human skin model.

Authors:  Olga Zlobinskaya; Stefanie Girst; Christoph Greubel; Volker Hable; Christian Siebenwirth; Dietrich W M Walsh; Gabriele Multhoff; Jan J Wilkens; Thomas E Schmid; Günther Dollinger
Journal:  Radiat Environ Biophys       Date:  2012-12-28       Impact factor: 1.925

8.  Design, Implementation, and in Vivo Validation of a Novel Proton FLASH Radiation Therapy System.

Authors:  Eric S Diffenderfer; Ioannis I Verginadis; Michele M Kim; Khayrullo Shoniyozov; Anastasia Velalopoulou; Denisa Goia; Mary Putt; Sarah Hagan; Stephen Avery; Kevin Teo; Wei Zou; Alexander Lin; Samuel Swisher-McClure; Cameron Koch; Ann R Kennedy; Andy Minn; Amit Maity; Theresa M Busch; Lei Dong; Costas Koumenis; James Metz; Keith A Cengel
Journal:  Int J Radiat Oncol Biol Phys       Date:  2020-02-01       Impact factor: 7.038

9.  Radiobiological influence of megavoltage electron pulses of ultra-high pulse dose rate on normal tissue cells.

Authors:  Lydia Laschinsky; Leonhard Karsch; Elisabeth Leßmann; Melanie Oppelt; Jörg Pawelke; Christian Richter; Michael Schürer; Elke Beyreuther
Journal:  Radiat Environ Biophys       Date:  2016-05-19       Impact factor: 1.925

10.  Biological effects in normal cells exposed to FLASH dose rate protons.

Authors:  Manuela Buonanno; Veljko Grilj; David J Brenner
Journal:  Radiother Oncol       Date:  2019-03-05       Impact factor: 6.280

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