Literature DB >> 202956

Transfer of ADP-ribose from NAD to choleragen: a subunit acts as catalyst and acceptor protein.

J B Trepel, D M Chuang, N H Neff.   

Abstract

Choleragen selectively incorporates 3H from [3H]NAD labeled on the adenosine moiety and not 14C from [14C]NAD labeled on the nicotinamide moiety. This reaction does not require protein in addition to choleragen. Incorporation of isotope does not proceed at 4 degrees, requires dithiothreitol, is stable after extensive washing with cold trichloroacetic acid, and is decreased 80% by boiling in trichloroacetic acid. Studies with the A and B subunits of choleragen show that the A subunit catalyzes ADP-ribosylation and serves as an acceptor protein. The B subunit does not show catalytic or acceptor activity. We conclude that choleragen and its A subunit catalyze the hydrolysis of NAD and the enzymatic transfer of ADP-ribose to the A subunit.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 202956      PMCID： PMC431754          DOI： 10.1073/pnas.74.12.5440

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Short communications. Subunit A from cholera toxin is an activator of adenylate cyclase in pigeon erythrocytes.

Authors:  S Van Heyningen; C A King
Journal:  Biochem J       Date:  1975-01       Impact factor: 3.857

2.  Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro.

Authors:  D M Gill
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Interaction of fragment A from diphtheria toxin with nicotinamide adenine dinucleotide.

Authors:  J Kandel; R J Collier; D W Chung
Journal:  J Biol Chem       Date:  1974-04-10       Impact factor: 5.157

5.  Adenosine diphosphate ribosylation of aminoacyl transferase II and inhibition of protein synthesis by diphtheria toxin.

Authors:  T Honjo; Y Nishizuka; I Kato; O Hayaishi
Journal:  J Biol Chem       Date:  1971-07-10       Impact factor: 5.157

6.  Gangliosides and membrane receptors for cholera toxin.

Authors:  P Cuatrecasas
Journal:  Biochemistry       Date:  1973-08-28       Impact factor: 3.162

7.  Small fragments from the A subunit of cholera toxin capable of activating adenylate cyclase.

Authors:  Y Matuo; M A Wheeler; M W Bitensky
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

8.  Dissociation and recombination of the subunits of the cholera enterotoxin (choleragen).

Authors:  R A Finkelstein; M Boesman; S H Neoh; M K LaRue; R Delaney
Journal:  J Immunol       Date:  1974-07       Impact factor: 5.422

9.  Hydrolysis of nicotinamide adenine dinucleotide by choleragen and its A protomer: possible role in the activation of adenylate cyclase.

Authors:  J Moss; V C Manganiello; M Vaughan
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

10.  Mechanism of action of choleragen. Evidence for ADP-ribosyltransferase activity with arginine as an acceptor.

Authors:  J Moss; M Vaughan
Journal:  J Biol Chem       Date:  1977-04-10       Impact factor: 5.157
View more
  11 in total

1.  ADP-ribosylation of membrane proteins catalyzed by cholera toxin: basis of the activation of adenylate cyclase.

Authors:  D M Gill; R Meren
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

2.  The adenylate cyclase-activating activity of cholera toxin is not associated with a nicotinamide--adenine dinucleotide glycohydrolase activity.

Authors:  R M Tait; S van Heyningen
Journal:  Biochem J       Date:  1978-09-15       Impact factor: 3.857

3.  Isolation of an avian erythrocyte protein possessing ADP-ribosyltransferase activity and capable of activating adenylate cyclase.

Authors:  J Moss; M Vaughan
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

4.  ADP-ribosylation factors: a family of approximately 20-kDa guanine nucleotide-binding proteins that activate cholera toxin.

Authors:  C F Welsh; J Moss; M Vaughan
Journal:  Mol Cell Biochem       Date:  1994-09       Impact factor: 3.396

Review 5.  Pathophysiological effects of Vibrio cholerae and enterotoxigenic Escherichia coli and their exotoxins on eucaryotic cells.

Authors:  K L Richards; S D Douglas
Journal:  Microbiol Rev       Date:  1978-09

6.  Mechanism of action of choleragen and E. coli heat-labile enterotoxin: activation of adenylate cyclase by ADP-ribosylation.

Authors:  J Moss; M Vaughan
Journal:  Mol Cell Biochem       Date:  1981-07-07       Impact factor: 3.396

7.  Characterization of heterotrimeric G-proteins in adult Acanthocheilonema viteae.

Authors:  K R Grant; M M Harnett; G Milligan; W Harnett
Journal:  Biochem J       Date:  1996-12-01       Impact factor: 3.857

8.  Requirement for guanosine triphosphate for cholera-toxin-catalysed incorporation of adenosine diphosphate ribose into rat liver plasma membranes and for activation of adenylate cyclase.

Authors:  C A Doberska; A J MacPherson; B R Martin
Journal:  Biochem J       Date:  1980-03-15       Impact factor: 3.857

9.  The A subunit of Escherichia coli heat-labile enterotoxin functions as a mucosal adjuvant and promotes IgG2a, IgA, and Th17 responses to vaccine antigens.

Authors:  Elizabeth B Norton; Louise B Lawson; Zaid Mahdi; Lucy C Freytag; John D Clements
Journal:  Infect Immun       Date:  2012-04-23       Impact factor: 3.441

Review 10.  Structure and function of cholera toxin and the related Escherichia coli heat-labile enterotoxin.

Authors:  B D Spangler
Journal:  Microbiol Rev       Date:  1992-12
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.