Literature DB >> 20217614

High-affinity immobilization of proteins using biotin- and GST-based coupling strategies.

Stephanie Q Hutsell1, Randall J Kimple, David P Siderovski, Francis S Willard, Adam J Kimple.   

Abstract

Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.

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Year:  2010        PMID： 20217614      PMCID： PMC3025018          DOI： 10.1007/978-1-60761-670-2_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation.

Authors:  D Beckett; E Kovaleva; P J Schatz
Journal:  Protein Sci       Date:  1999-04       Impact factor: 6.725

2.  AVIDIN. 1. THE USE OF (14-C)BIOTIN FOR KINETIC STUDIES AND FOR ASSAY.

Authors:  N M GREEN
Journal:  Biochem J       Date:  1963-12       Impact factor: 3.857

3.  AVIDIN. 2. PURIFICATION AND COMPOSITION.

Authors:  M D MELAMED; N M GREEN
Journal:  Biochem J       Date:  1963-12       Impact factor: 3.857

4.  Differential G-alpha interaction capacities of the GoLoco motifs in Rap GTPase activating proteins.

Authors:  Francis S Willard; Aaron B Low; Christopher R McCudden; David P Siderovski
Journal:  Cell Signal       Date:  2006-09-01       Impact factor: 4.315

5.  Covalent immobilization of histidine-tagged proteins for surface plasmon resonance.

Authors:  Francis S Willard; David P Siderovski
Journal:  Anal Biochem       Date:  2006-02-23       Impact factor: 3.365

6.  Minimal determinants for binding activated G alpha from the structure of a G alpha(i1)-peptide dimer.

Authors:  Christopher A Johnston; Ekaterina S Lobanova; Alexander S Shavkunov; Justin Low; J Kevin Ramer; Rainer Blaesius; Zoey Fredericks; Francis S Willard; Brian Kuhlman; Vadim Y Arshavsky; David P Siderovski
Journal:  Biochemistry       Date:  2006-09-26       Impact factor: 3.162

7.  A point mutation to Galphai selectively blocks GoLoco motif binding: direct evidence for Galpha.GoLoco complexes in mitotic spindle dynamics.

Authors:  Francis S Willard; Zhen Zheng; Juan Guo; Gregory J Digby; Adam J Kimple; Jason M Conley; Christopher A Johnston; Dustin Bosch; Melinda D Willard; Val J Watts; Nevin A Lambert; Stephen R Ikeda; Quansheng Du; David P Siderovski
Journal:  J Biol Chem       Date:  2008-11-04       Impact factor: 5.157

Review 8.  Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins.

Authors:  M Malmqvist; R Karlsson
Journal:  Curr Opin Chem Biol       Date:  1997-10       Impact factor: 8.822

9.  A direct fluorescence-based assay for RGS domain GTPase accelerating activity.

Authors:  Francis S Willard; Adam J Kimple; Christopher A Johnston; David P Siderovski
Journal:  Anal Biochem       Date:  2005-05-15       Impact factor: 3.365

10.  Kinetic analysis of high-mobility-group proteins HMG-1 and HMG-I/Y binding to cholesterol-tagged DNA on a supported lipid monolayer.

Authors:  C I Webster; M A Cooper; L C Packman; D H Williams; J C Gray
Journal:  Nucleic Acids Res       Date:  2000-04-01       Impact factor: 16.971
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  16 in total

1.  Structural determinants of affinity enhancement between GoLoco motifs and G-protein alpha subunit mutants.

Authors:  Dustin E Bosch; Adam J Kimple; Deanne W Sammond; Robin E Muller; Michael J Miley; Mischa Machius; Brian Kuhlman; Francis S Willard; David P Siderovski
Journal:  J Biol Chem       Date:  2010-11-29       Impact factor: 5.157

Review 2.  Enzyme immobilization: an update.

Authors:  Ahmad Abolpour Homaei; Reyhaneh Sariri; Fabio Vianello; Roberto Stevanato
Journal:  J Chem Biol       Date:  2013-08-29

3.  Secondary modification of oxidatively-modified proline N-termini for the construction of complex bioconjugates.

Authors:  Johnathan C Maza; Alexandra V Ramsey; Meire Mehare; Shane W Krska; Craig A Parish; Matthew B Francis
Journal:  Org Biomol Chem       Date:  2020-02-26       Impact factor: 3.876

Review 4.  In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.

Authors:  Pedro Jimenez Sandoval; Julia Santiago
Journal:  Plant Physiol       Date:  2020-02-07       Impact factor: 8.340

5.  On-chip bioorthogonal chemistry enables immobilization of in situ modified nanoparticles and small molecules for label-free monitoring of protein binding and reaction kinetics.

Authors:  C Tassa; M Liong; S Hilderbrand; J E Sandler; T Reiner; E J Keliher; R Weissleder; S Y Shaw
Journal:  Lab Chip       Date:  2012-07-03       Impact factor: 6.799

6.  Regulator of G-protein signaling-21 (RGS21) is an inhibitor of bitter gustatory signaling found in lingual and airway epithelia.

Authors:  Staci P Cohen; Brian K Buckley; Mickey Kosloff; Alaina L Garland; Dustin E Bosch; Gang Cheng; Harish Radhakrishna; Michael D Brown; Francis S Willard; Vadim Y Arshavsky; Robert Tarran; David P Siderovski; Adam J Kimple
Journal:  J Biol Chem       Date:  2012-10-24       Impact factor: 5.157

7.  Expression, isolation, and purification of soluble and insoluble biotinylated proteins for nerve tissue regeneration.

Authors:  Aleesha M McCormick; Natalie A Jarmusik; Elizabeth J Endrizzi; Nic D Leipzig
Journal:  J Vis Exp       Date:  2014-01-22       Impact factor: 1.355

8.  Functional and Structural Characterization of Bub3·BubR1 Interactions Required for Spindle Assembly Checkpoint Signaling in Human Cells.

Authors:  Florian Prinz; Vera Puetter; Simon J Holton; Dorothee Andres; Christian M Stegmann; Dennis Kwiatkowski; Stefan Prechtl; Kirstin Petersen; Georg Beckmann; Bertolt Kreft; Dominik Mumberg; Amaury Fernández-Montalván
Journal:  J Biol Chem       Date:  2016-03-30       Impact factor: 5.157

9.  Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis.

Authors:  Dustin E Bosch; Adam J Kimple; Alyssa J Manning; Robin E Muller; Francis S Willard; Mischa Machius; Stephen L Rogers; David P Siderovski
Journal:  Structure       Date:  2012-12-20       Impact factor: 5.006

Review 10.  Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics.

Authors:  Marcin Drozd; Sylwia Karoń; Elżbieta Malinowska
Journal:  Sensors (Basel)       Date:  2021-05-29       Impact factor: 3.576
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