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Literature DB >> 20199060

Acoustophoretic synchronization of mammalian cells in microchannels.

Patrick Thévoz¹, Jonathan D Adams, Herbert Shea, Henrik Bruus, H Tom Soh.

Abstract

We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G(1) phase synchrony (approximately 84%) and throughput (3 x 10(6) cells/h per microchannel).

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：
Propidium

Year: 2010 PMID： 20199060 PMCID： PMC2848285 DOI： 10.1021/ac100357u

Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986

31 in total

Review 1. Rethinking synchronization of mammalian cells for cell cycle analysis.

Authors: S Cooper
Journal: Cell Mol Life Sci Date: 2003-06 Impact factor: 9.261

2. Acoustic control of suspended particles in micro fluidic chips.

Authors: Andreas Nilsson; Filip Petersson; Henrik Jönsson; Thomas Laurell
Journal: Lab Chip Date: 2004-02-09 Impact factor: 6.799

Review 3. How cells coordinate growth and division.

Authors: Paul Jorgensen; Mike Tyers
Journal: Curr Biol Date: 2004-12-14 Impact factor: 10.834

4. Continuous separation of lipid particles from erythrocytes by means of laminar flow and acoustic standing wave forces.

Authors: Filip Petersson; Andreas Nilsson; Cecilia Holm; Henrik Jonsson; Thomas Laurell
Journal: Lab Chip Date: 2004-09-17 Impact factor: 6.799

5. Chip integrated strategies for acoustic separation and manipulation of cells and particles.

Authors: Thomas Laurell; Filip Petersson; Andreas Nilsson
Journal: Chem Soc Rev Date: 2006-12-07 Impact factor: 54.564

6. Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis.

Authors: Unyoung Kim; Chih-Wen Shu; Karen Y Dane; Patrick S Daugherty; Jean Y J Wang; H T Soh
Journal: Proc Natl Acad Sci U S A Date: 2007-12-19 Impact factor: 11.205

7. Free flow acoustophoresis: microfluidic-based mode of particle and cell separation.

Authors: Filip Petersson; Lena Aberg; Ann-Margret Swärd-Nilsson; Thomas Laurell
Journal: Anal Chem Date: 2007-06-15 Impact factor: 6.986

8. Cell cycle regulation of metallothionein in human colonic cancer cells.

Authors: W W Nagel; B L Vallee
Journal: Proc Natl Acad Sci U S A Date: 1995-01-17 Impact factor: 11.205

9. Growth-regulatory properties of G1 cells synchronized by centrifugal elutriation.

Authors: P Zickert; J Wejde; S Skog; A Zetterberg; O Larsson
Journal: Exp Cell Res Date: 1993-07 Impact factor: 3.905

10. Cell cycle- and growth phase-dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures.

Authors: O T Ramirez; R Mutharasan
Journal: Biotechnol Bioeng Date: 1990-10-20 Impact factor: 4.530

22 in total

Acoustophoretic synchronization of mammalian cells in microchannels.

Review 1. Rethinking synchronization of mammalian cells for cell cycle analysis.

2. Acoustic control of suspended particles in micro fluidic chips.

Review 3. How cells coordinate growth and division.

4. Continuous separation of lipid particles from erythrocytes by means of laminar flow and acoustic standing wave forces.

5. Chip integrated strategies for acoustic separation and manipulation of cells and particles.

6. Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis.

7. Free flow acoustophoresis: microfluidic-based mode of particle and cell separation.

8. Cell cycle regulation of metallothionein in human colonic cancer cells.

9. Growth-regulatory properties of G1 cells synchronized by centrifugal elutriation.

10. Cell cycle- and growth phase-dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures.

1. Tunable acoustophoretic band-pass particle sorter.

2. Clinical-Scale Cell-Surface-Marker Independent Acoustic Microfluidic Enrichment of Tumor Cells from Blood.

3. Concurrent isolation of lymphocytes and granulocytes using prefocused free flow acoustophoresis.

4. Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift.

5. The assembly of cell-encapsulating microscale hydrogels using acoustic waves.

Review 6. SAW-driven droplet jetting technology in microfluidic: A review.

7. Isolating single cells in a neurosphere assay using inertial microfluidics.

8. Thermally-assisted ultrasonic separation of giant vesicles.

9. Acoustofluidic methods in cell analysis.

10. Acoustophoretic sorting of viable mammalian cells in a microfluidic device.