| Literature DB >> 20188721 |
Hiba Tannoury1, Varenka Rodriguez, Ismar Kovacevic, Mouna Ibourk, Myeongwoo Lee, Erin J Cram.
Abstract
The two specialized C. elegans distal tip cells (DTCs) provide an in vivo model system for the study of developmentally regulated cell migration. We identified cacn-1/cactin, a well-conserved, novel regulator of cell migration in a genome-wide RNAi screen for regulators of DTC migration. RNAi depletion experiments and analysis of the hypomorphic allele cacn-1(tm3126) indicate that CACN-1 is required during DTC migration for proper pathfinding and for cessation of DTC migration at the end of larval morphogenesis. Strong expression of CACN-1 in the DTCs, and data from cell-specific RNAi depletion experiments, suggest that CACN-1 is required cell-autonomously to control DTC migration. Importantly, genetic interaction data with Rac GTPase activators and effectors suggest that CACN-1 acts specifically to inhibit the mig-2/Rac pathway, and in parallel to ced-10/Rac, to control DTC pathfinding. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20188721 PMCID： PMC2854247 DOI： 10.1016/j.ydbio.2010.02.025
Source DB: PubMed Journal: Dev Biol ISSN： 0012-1606 Impact factor: 3.582