Literature DB >> 20181898

Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.

Pavel Drevinek1, Sarka Vosahlikova, Klara Dedeckova, Ondrej Cinek, Eshwar Mahenthiralingam.   

Abstract

We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.

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Year:  2010        PMID： 20181898      PMCID： PMC2863923          DOI： 10.1128/JCM.02359-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA.

Authors:  Pavel Drevínek; Hana Hrbácková; Ondrej Cinek; Jana Bartosová; Otakar Nyc; Alexandr Nemec; Petr Pohunek
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

2.  DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III.

Authors:  E Mahenthiralingam; J Bischof; S K Byrne; C Radomski; J E Davies; Y Av-Gay; P Vandamme
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

3.  Evidence of coinfection with distinct strains of Burkholderia multivorans in a cystic fibrosis patient.

Authors:  N Wellinghausen; J Köthe
Journal:  Infection       Date:  2006-10       Impact factor: 3.553

4.  Improved molecular detection of Burkholderia cepacia genomovar III and Burkholderia multivorans directly from sputum of patients with cystic fibrosis.

Authors:  John E Moore; Jiru Xu; Beverley C Millar; Mary Crowe; J Stuart Elborn
Journal:  J Microbiol Methods       Date:  2002-04       Impact factor: 2.363

5.  Expanded multilocus sequence typing for burkholderia species.

Authors:  Theodore Spilker; Adam Baldwin; Amy Bumford; Chris G Dowson; Eshwar Mahenthiralingam; John J LiPuma
Journal:  J Clin Microbiol       Date:  2009-06-03       Impact factor: 5.948

6.  Cross-sectional and longitudinal multilocus sequence typing of pseudomonas aeruginosa in cystic fibrosis sputum samples.

Authors:  David J Waine; David Honeybourne; E Grace Smith; Joanna L Whitehouse; Chris G Dowson
Journal:  J Clin Microbiol       Date:  2009-08-26       Impact factor: 5.948

7.  Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.

Authors:  Elke Vanlaere; John J Lipuma; Adam Baldwin; Deborah Henry; Evie De Brandt; Eshwar Mahenthiralingam; David Speert; Chris Dowson; Peter Vandamme
Journal:  Int J Syst Evol Microbiol       Date:  2008-07       Impact factor: 2.747

8.  RecA gene sequence and Multilocus Sequence Typing for species-level resolution of Burkholderia cepacia complex isolates.

Authors:  S Cesarini; A Bevivino; S Tabacchioni; L Chiarini; C Dalmastri
Journal:  Lett Appl Microbiol       Date:  2009-08-07       Impact factor: 2.858

9.  Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.

Authors:  Elke Vanlaere; Adam Baldwin; Dirk Gevers; Deborah Henry; Evie De Brandt; John J LiPuma; Eshwar Mahenthiralingam; David P Speert; Chris Dowson; Peter Vandamme
Journal:  Int J Syst Evol Microbiol       Date:  2009-01       Impact factor: 2.747

10.  Multilocus sequence typing breathes life into a microbial metagenome.

Authors:  Eshwar Mahenthiralingam; Adam Baldwin; Pavel Drevinek; Elke Vanlaere; Peter Vandamme; John J LiPuma; Chris G Dowson
Journal:  PLoS One       Date:  2006-12-20       Impact factor: 3.240
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  4 in total

1.  Rapid Detection of Emerging Pathogens and Loss of Microbial Diversity Associated with Severe Lung Disease in Cystic Fibrosis.

Authors:  William G Flight; Ann Smith; Christopher Paisey; Julian R Marchesi; Matthew J Bull; Phillip J Norville; Ken J Mutton; A Kevin Webb; Rowland J Bright-Thomas; Andrew M Jones; Eshwar Mahenthiralingam
Journal:  J Clin Microbiol       Date:  2015-04-15       Impact factor: 5.948

2.  Burkholderia cepacia Complex: Emerging Multihost Pathogens Equipped with a Wide Range of Virulence Factors and Determinants.

Authors:  Sílvia A Sousa; Christian G Ramos; Jorge H Leitão
Journal:  Int J Microbiol       Date:  2010-08-03

3.  Polyclonal outbreak of bloodstream infections caused by Burkholderia cepacia complex in hematology and bone marrow transplant outpatient units.

Authors:  Icaro Boszczowski; Gladys Villas Boas do Prado; Mirian F Dalben; Roberto C P Telles; Maristela Pinheiro Freire; Thaís Guimarães; Maura S Oliveira; Juliana F Rosa; Robson E Soares; Pedro Enrique Dorlhiac Llacer; Frederico Luiz Dulley; Silvia F Costa; Anna S Levin
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2014 Jan-Feb       Impact factor: 1.846

4.  Evaluation of Three Culture Media for Isolation of Burkholderia cepacia Complex from Respiratory Samples of Patients with Cystic Fibrosis.

Authors:  Emma C L Marrs; Audrey Perry; John D Perry
Journal:  Microorganisms       Date:  2021-12-16
  4 in total

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