AIM: To identify, by means of recA sequencing and multilocus sequence typing (MLST), Burkholderia cepacia complex (BCC) isolates of environmental and clinical origin, which failed to be identified by recA RFLP and species-specific PCR. METHODS AND RESULTS: By using recA sequence-based identification, 17 out of 26 BCC isolates were resolved at the level of species and lineage (ten Burkholderia cenocepacia IIIB, two Burkholderia arboris and five Burkholderia lata). By using MLST method, 24 BCC isolates were identified. MLST confirmed recA sequence results, and, furthermore, enabled to identify isolates of the BCC5 group, and showed relatedness with Burkholderia contaminans for one of the two isolates not identified. CONCLUSIONS: recA sequence-based identification allowed to resolve, at the level of species and lineage, 65.4%, of the BCC isolates examined, whilst MLST increased this percentage to 88.5%. SIGNIFICANCE AND IMPACT OF THE STUDY: BCC isolates previously not resolved by recA RFLP and species-specific PCR were successfully identified by means of recA sequencing and MLST, which represent the most appropriate methods to identify difficult strains for epidemiological purposes and cystic fibrosis patients management.
AIM: To identify, by means of recA sequencing and multilocus sequence typing (MLST), Burkholderia cepacia complex (BCC) isolates of environmental and clinical origin, which failed to be identified by recA RFLP and species-specific PCR. METHODS AND RESULTS: By using recA sequence-based identification, 17 out of 26 BCC isolates were resolved at the level of species and lineage (ten Burkholderia cenocepacia IIIB, two Burkholderia arboris and five Burkholderia lata). By using MLST method, 24 BCC isolates were identified. MLST confirmed recA sequence results, and, furthermore, enabled to identify isolates of the BCC5 group, and showed relatedness with Burkholderia contaminans for one of the two isolates not identified. CONCLUSIONS:recA sequence-based identification allowed to resolve, at the level of species and lineage, 65.4%, of the BCC isolates examined, whilst MLST increased this percentage to 88.5%. SIGNIFICANCE AND IMPACT OF THE STUDY: BCC isolates previously not resolved by recA RFLP and species-specific PCR were successfully identified by means of recA sequencing and MLST, which represent the most appropriate methods to identify difficult strains for epidemiological purposes and cystic fibrosispatients management.
Authors: R Manji; M Bythrow; J A Branda; C-A D Burnham; M J Ferraro; O B Garner; R Jennemann; M A Lewinski; A B Mochon; G W Procop; S S Richter; J A Rychert; L Sercia; L F Westblade; C C Ginocchio Journal: Eur J Clin Microbiol Infect Dis Date: 2013-09-10 Impact factor: 3.267
Authors: T Mann; D Ben-David; A Zlotkin; D Shachar; N Keller; A Toren; A Nagler; G Smollan; A Barzilai; G Rahav Journal: Infection Date: 2010-04-01 Impact factor: 3.553
Authors: Rachel F Power; Barry Linnane; Ruth Martin; Noelle Power; Peig Harnett; Brian Casserly; Nuala H O'Connell; Colum P Dunne Journal: BMC Pulm Med Date: 2016-04-22 Impact factor: 3.317
Authors: Melanie C Melendrez; Eric D Becraft; Jason M Wood; Millie T Olsen; Donald A Bryant; John F Heidelberg; Douglas B Rusch; Frederick M Cohan; David M Ward Journal: Front Microbiol Date: 2016-01-14 Impact factor: 5.640
Authors: Carina M Hall; Joseph D Busch; Kenzie Shippy; Christopher J Allender; Mirjam Kaestli; Mark Mayo; Jason W Sahl; James M Schupp; Rebecca E Colman; Paul Keim; Bart J Currie; David M Wagner Journal: PLoS One Date: 2015-11-23 Impact factor: 3.240