Literature DB >> 12202599

Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA.

Pavel Drevínek1, Hana Hrbácková, Ondrej Cinek, Jana Bartosová, Otakar Nyc, Alexandr Nemec, Petr Pohunek.   

Abstract

We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

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Year:  2002        PMID： 12202599      PMCID： PMC130775          DOI： 10.1128/JCM.40.9.3485-3488.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex.

Authors:  E Mahenthiralingam; T Coenye; J W Chung; D P Speert; J R Govan; P Taylor; P Vandamme
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex.

Authors:  D A Henry; E Mahenthiralingam; P Vandamme; T Coenye; D P Speert
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

Review 3.  Taxonomy and identification of the Burkholderia cepacia complex.

Authors:  T Coenye; P Vandamme; J R Govan; J J LiPuma
Journal:  J Clin Microbiol       Date:  2001-10       Impact factor: 5.948

4.  Disproportionate distribution of Burkholderia cepacia complex species and transmissibility markers in cystic fibrosis.

Authors:  J J LiPuma; T Spilker; L H Gill; P W Campbell; L Liu; E Mahenthiralingam
Journal:  Am J Respir Crit Care Med       Date:  2001-07-01       Impact factor: 21.405

Review 5.  Unusual respiratory bacterial flora in cystic fibrosis: microbiologic and clinical features.

Authors:  P M Beringer; M D Appleman
Journal:  Curr Opin Pulm Med       Date:  2000-11       Impact factor: 3.155

6.  Burkholderia cepacia complex infection in Italian patients with cystic fibrosis: prevalence, epidemiology, and genomovar status.

Authors:  A Agodi; E Mahenthiralingam; M Barchitta; V Gianninò; A Sciacca; S Stefani
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

7.  DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III.

Authors:  E Mahenthiralingam; J Bischof; S K Byrne; C Radomski; J E Davies; Y Av-Gay; P Vandamme
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

Review 8.  Burkholderia cepacia: current clinical issues, environmental controversies and ethical dilemmas.

Authors:  A M Jones; M E Dodd; A K Webb
Journal:  Eur Respir J       Date:  2001-02       Impact factor: 16.671

9.  Infection with Burkholderia cepacia complex genomovars in patients with cystic fibrosis: virulent transmissible strains of genomovar III can replace Burkholderia multivorans.

Authors:  E Mahenthiralingam; P Vandamme; M E Campbell; D A Henry; A M Gravelle; L T Wong; A G Davidson; P G Wilcox; B Nakielna; D P Speert
Journal:  Clin Infect Dis       Date:  2001-10-04       Impact factor: 9.079

10.  Multiple combination bactericidal antibiotic testing for patients with cystic fibrosis infected with Burkholderia cepacia.

Authors:  S D Aaron; W Ferris; D A Henry; D P Speert; N E Macdonald
Journal:  Am J Respir Crit Care Med       Date:  2000-04       Impact factor: 21.405
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  7 in total

Review 1.  Laboratory aspects of management of chronic pulmonary infections in patients with cystic fibrosis.

Authors:  Melissa B Miller; Peter H Gilligan
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

2.  Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.

Authors:  Pavel Drevinek; Sarka Vosahlikova; Klara Dedeckova; Ondrej Cinek; Eshwar Mahenthiralingam
Journal:  J Clin Microbiol       Date:  2010-02-24       Impact factor: 5.948

3.  Differentiation of species combined into the Burkholderia cepacia complex and related taxa on the basis of their fatty acid patterns.

Authors:  Eva Krejcí; Reiner M Kroppenstedt
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

4.  Microbiology of airway disease in a cohort of patients with cystic fibrosis.

Authors:  Antonietta Lambiase; Valeria Raia; Mariassunta Del Pezzo; Angela Sepe; Vincenzo Carnovale; Fabio Rossano
Journal:  BMC Infect Dis       Date:  2006-01-11       Impact factor: 3.090

5.  Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia.

Authors:  Pavel Drevinek; Adam Baldwin; Christopher G Dowson; Eshwar Mahenthiralingam
Journal:  BMC Microbiol       Date:  2008-03-07       Impact factor: 3.605

6.  PCR detection of Burkholderia multivorans in water and soil samples.

Authors:  Charlotte Peeters; Stijn Daenekindt; Peter Vandamme
Journal:  BMC Microbiol       Date:  2016-08-12       Impact factor: 3.605

7.  Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients.

Authors:  Luana Pretto; Fernanda de-Paris; Alice Beatriz Mombach Pinheiro Machado; Andreza Francisco Martins; Afonso Luís Barth
Journal:  Braz J Infect Dis       Date:  2013-01-01       Impact factor: 3.257
  7 in total

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