BACKGROUND: Ventricular tachycardia (VT) is a common cause of mortality in post-myocardial infarction (MI) patients, even in the current era of coronary revascularization treatment. We report a reproducible VT model in rats with chronic MI induced by ischemia-reperfusion and describe its electrophysiological characteristics using high-resolution optical mapping. METHODS: An MI was generated by left anterior descending coronary ligation (25 minutes) followed by reperfusion in 20 rats. Electrophysiology study and optical mapping were performed 5 weeks later using a Langendorff-perfused preparation and compared to normal rats. RESULTS: The conduction velocity of the MI border zone was decreased to 53% of the normal areas remote from the infarct (0.37 +/- 0.16 m/sec vs 0.70 +/- 0.09 m/sec, P < 0.0001). The rate of VT inducibility in MI rats was significantly greater than in normal control rats (70% vs 0%, P = 0.00002). VT circuits involving the infarct area were identified with optical mapping in 83% MI rats. In addition, fixed and functional conduction block were observed in the infarct border zone. CONCLUSION: This ischemia-reperfusion MI rat model is a reliable VT model, which simulates clinical revascularization treatment. High-resolution optical mapping in this model is useful to study the mechanism of VT and evaluate the effects of therapies.
BACKGROUND:Ventricular tachycardia (VT) is a common cause of mortality in post-myocardial infarction (MI) patients, even in the current era of coronary revascularization treatment. We report a reproducible VT model in rats with chronic MI induced by ischemia-reperfusion and describe its electrophysiological characteristics using high-resolution optical mapping. METHODS: An MI was generated by left anterior descending coronary ligation (25 minutes) followed by reperfusion in 20 rats. Electrophysiology study and optical mapping were performed 5 weeks later using a Langendorff-perfused preparation and compared to normal rats. RESULTS: The conduction velocity of the MI border zone was decreased to 53% of the normal areas remote from the infarct (0.37 +/- 0.16 m/sec vs 0.70 +/- 0.09 m/sec, P < 0.0001). The rate of VT inducibility in MI rats was significantly greater than in normal control rats (70% vs 0%, P = 0.00002). VT circuits involving the infarct area were identified with optical mapping in 83% MI rats. In addition, fixed and functional conduction block were observed in the infarct border zone. CONCLUSION: This ischemia-reperfusion MI rat model is a reliable VT model, which simulates clinical revascularization treatment. High-resolution optical mapping in this model is useful to study the mechanism of VT and evaluate the effects of therapies.
Authors: Jacob I Laughner; Fu Siong Ng; Matthew S Sulkin; R Martin Arthur; Igor R Efimov Journal: Am J Physiol Heart Circ Physiol Date: 2012-07-20 Impact factor: 4.733
Authors: Peter Lee; Fouad Taghavi; Ping Yan; Paul Ewart; Euan A Ashley; Leslie M Loew; Peter Kohl; Christian Bollensdorff; Christopher E Woods Journal: PLoS One Date: 2012-08-02 Impact factor: 3.240
Authors: Fu Siong Ng; Jeremy M Kalindjian; Simon A Cooper; Rasheda A Chowdhury; Pravina M Patel; Emmanuel Dupont; Alexander R Lyon; Nicholas S Peters Journal: JACC Clin Electrophysiol Date: 2016-10
Authors: Dominic Filice; Wahiba Dhahri; Joell L Solan; Paul D Lampe; Erin Steele; Nikita Milani; Benjamin Van Biber; Wei-Zhong Zhu; Tamilla Sadikov Valdman; Rocco Romagnuolo; José David Otero-Cruz; Kip D Hauch; Matthew W Kay; Narine Sarvazyan; Michael A Laflamme Journal: Stem Cell Res Ther Date: 2020-09-25 Impact factor: 6.832
Authors: Sophia L Suarez; Aboli A Rane; Adam Muñoz; Adam T Wright; Shirley X Zhang; Rebecca L Braden; Adah Almutairi; Andrew D McCulloch; Karen L Christman Journal: Acta Biomater Date: 2015-08-08 Impact factor: 8.947