Literature DB >> 20173074

New efficient recombinant expression system to engineer Candida antarctica lipase B.

Stéphane Emond1, Cédric Montanier, Jean-Marc Nicaud, Alain Marty, Pierre Monsan, Isabelle André, Magali Remaud-Siméon.   

Abstract

Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.

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Year:  2010        PMID： 20173074      PMCID： PMC2849202          DOI： 10.1128/AEM.03057-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  23 in total

1.  Protein library design and screening: working out the probabilities.

Authors:  Michel Denault; Joelle N Pelletier
Journal:  Methods Mol Biol       Date:  2007

2.  Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from Candida antarctica.

Authors:  Sriappareddy Tamalampudi; Md Mahabubur Rahman Talukder; Shinji Hama; Takanori Tanino; Yuya Suzuki; Akihiko Kondo; Hideki Fukuda
Journal:  Appl Microbiol Biotechnol       Date:  2007-01-16       Impact factor: 4.813

3.  Investigating the structural and functional consequences of circular permutation on lipase B from Candida antarctica.

Authors:  Zhen Qian; Christina J Fields; Stefan Lutz
Journal:  Chembiochem       Date:  2007-11-05       Impact factor: 3.164

Review 4.  Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis.

Authors:  Manfred T Reetz; Daniel Kahakeaw; Joaquin Sanchis
Journal:  Mol Biosyst       Date:  2008-12-09

5.  Understanding the plasticity of the alpha/beta hydrolase fold: lid swapping on the Candida antarctica lipase B results in chimeras with interesting biocatalytic properties.

Authors:  Michael Skjøt; Leonardo De Maria; Robin Chatterjee; Allan Svendsen; Shamkant A Patkar; Peter R Ostergaard; Jesper Brask
Journal:  Chembiochem       Date:  2009-02-13       Impact factor: 3.164

6.  Finding better protein engineering strategies.

Authors:  Romas J Kazlauskas; Uwe T Bornscheuer
Journal:  Nat Chem Biol       Date:  2009-08       Impact factor: 15.040

7.  Addressing the numbers problem in directed evolution.

Authors:  Manfred T Reetz; Daniel Kahakeaw; Renate Lohmer
Journal:  Chembiochem       Date:  2008-07-21       Impact factor: 3.164

8.  Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems.

Authors:  Marianne Wittrup Larsen; Uwe T Bornscheuer; Karl Hult
Journal:  Protein Expr Purif       Date:  2008-08-07       Impact factor: 1.650

9.  Functional fusion mutant of Candida antarctica lipase B (CalB) expressed in Escherichia coli.

Authors:  Hyuk-Seong Seo; Seong-Eun Kim; Kyung-Yeon Han; Jin-Seung Park; Yong-Hwan Kim; Sang Jun Sim; Jeewon Lee
Journal:  Biochim Biophys Acta       Date:  2008-12-24

10.  In vitro evolution of lipase B from Candida antarctica using surface display in hansenula polymorpha.

Authors:  So-Young Kim; Jung-Hoon Sohn; Yu-Ryang Pyun; In Seok Yang; Kyung-Hyun Kim; Eui-Sung Choi
Journal:  J Microbiol Biotechnol       Date:  2007-08       Impact factor: 2.351
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  7 in total

Review 1.  Adaptational properties and applications of cold-active lipases from psychrophilic bacteria.

Authors:  Jonathan Maiangwa; Mohd Shukuri Mohamad Ali; Abu Bakar Salleh; Raja Noor Zaliha Raja Abd Rahman; Fairolniza Mohd Shariff; Thean Chor Leow
Journal:  Extremophiles       Date:  2014-12-04       Impact factor: 2.395

Review 2.  Thermostable lipases and their dynamics of improved enzymatic properties.

Authors:  Siti Hajar Hamdan; Jonathan Maiangwa; Mohd Shukuri Mohamad Ali; Yahaya M Normi; Suriana Sabri; Thean Chor Leow
Journal:  Appl Microbiol Biotechnol       Date:  2021-09-06       Impact factor: 5.560

Review 3.  Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up.

Authors:  Aravind Madhavan; K B Arun; Raveendran Sindhu; Jayaram Krishnamoorthy; R Reshmy; Ranjna Sirohi; Arivalagan Pugazhendi; Mukesh Kumar Awasthi; George Szakacs; Parameswaran Binod
Journal:  Microb Cell Fact       Date:  2021-06-30       Impact factor: 5.328

4.  Streamlined Preparation of Immobilized Candida antarctica Lipase B.

Authors:  Karim Engelmark Cassimjee; Peter Hendil-Forssell; Alexey Volkov; Anne Krog; Jostein Malmo; Trond Erik V Aune; Wolfgang Knecht; Iain R Miskelly; Thomas S Moody; Maria Svedendahl Humble
Journal:  ACS Omega       Date:  2017-12-06

5.  Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions.

Authors:  Young-Kyoung Park; Marie Vandermies; Paul Soudier; Samuel Telek; Stéphane Thomas; Jean-Marc Nicaud; Patrick Fickers
Journal:  Microb Cell Fact       Date:  2019-10-10       Impact factor: 5.328

6.  Molecular engineering of fungal GH5 and GH26 beta-(1,4)-mannanases toward improvement of enzyme activity.

Authors:  Marie Couturier; Julia Féliu; Sophie Bozonnet; Alain Roussel; Jean-Guy Berrin
Journal:  PLoS One       Date:  2013-11-22       Impact factor: 3.240

Review 7.  Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica.

Authors:  Marie Vandermies; Patrick Fickers
Journal:  Microorganisms       Date:  2019-01-30
  7 in total

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