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Literature DB >> 19620988

Finding better protein engineering strategies.

Romas J Kazlauskas¹, Uwe T Bornscheuer.

Abstract

Mesh：

Substances：
Proteins

Year: 2009 PMID： 19620988 DOI： 10.1038/nchembio0809-526

Source DB: PubMed Journal: Nat Chem Biol ISSN： 1552-4450 Impact factor: 15.040

× No keyword cloud information.

22 in total

1. Enhancement, relaxation, and reversal of the stereoselectivity for phosphotriesterase by rational evolution of active site residues.

Authors: M Chen-Goodspeed; M A Sogorb; F Wu; F M Raushel
Journal: Biochemistry Date: 2001-02-06 Impact factor: 3.162

2. Metabolix, Inc. and Tepha, Inc. Bioplastics for industry and medical devices.

Authors: Alice McCarthy
Journal: Chem Biol Date: 2003-10

3. Improving the catalytic activity of Candida antarctica lipase B by circular permutation.

Authors: Zhen Qian; Stefan Lutz
Journal: J Am Chem Soc Date: 2005-10-05 Impact factor: 15.419

4. Directed enzyme evolution via small and effective neutral drift libraries.

Authors: Rinkoo D Gupta; Dan S Tawfik
Journal: Nat Methods Date: 2008-10-19 Impact factor: 28.547

5. Engineering delta 9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor delta 9-18:0-ACP desaturase.

Authors: E Whittle; J Shanklin
Journal: J Biol Chem Date: 2001-04-09 Impact factor: 5.157

6. Improving catalytic function by ProSAR-driven enzyme evolution.

Authors: Richard J Fox; S Christopher Davis; Emily C Mundorff; Lisa M Newman; Vesna Gavrilovic; Steven K Ma; Loleta M Chung; Charlene Ching; Sarena Tam; Sheela Muley; John Grate; John Gruber; John C Whitman; Roger A Sheldon; Gjalt W Huisman
Journal: Nat Biotechnol Date: 2007-02-18 Impact factor: 54.908

7. Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).

Authors: Grace DeSantis; Kelvin Wong; Bob Farwell; Kelly Chatman; Zoulin Zhu; Geoff Tomlinson; Hongjun Huang; Xuqiu Tan; Lisa Bibbs; Pei Chen; Keith Kretz; Mark J Burk
Journal: J Am Chem Soc Date: 2003-09-24 Impact factor: 15.419

Review 8. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.

Authors: Cameron Neylon
Journal: Nucleic Acids Res Date: 2004-02-27 Impact factor: 16.971

9. Selection and evolution of enzymes from a partially randomized non-catalytic scaffold.

Authors: Burckhard Seelig; Jack W Szostak
Journal: Nature Date: 2007-08-16 Impact factor: 49.962

10. Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution.

Authors: Jesse D Bloom; Philip A Romero; Zhongyi Lu; Frances H Arnold
Journal: Biol Direct Date: 2007-06-28 Impact factor: 4.540

50 in total

1. Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.

Authors: Anders G Sandström; Ylva Wikmark; Karin Engström; Jonas Nyhlén; Jan-E Bäckvall
Journal: Proc Natl Acad Sci U S A Date: 2011-12-16 Impact factor: 11.205

2. Crystallization and preliminary crystallographic analysis of LipC12, a true lipase isolated through a metagenomics approach.

Authors: V P Martini; A Glogauer; J Iulek; E M Souza; F O Pedrosa; N Krieger
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2012-01-26

10. Fusion of an oligopeptide to the N terminus of an alkaline α-amylase from Alkalimonas amylolytica simultaneously improves the enzyme's catalytic efficiency, thermal stability, and resistance to oxidation.

Authors: Haiquan Yang; Xinyao Lu; Long Liu; Jianghua Li; Hyun-dong Shin; Rachel R Chen; Guocheng Du; Jian Chen
Journal: Appl Environ Microbiol Date: 2013-03-01 Impact factor: 4.792

Finding better protein engineering strategies.

1. Enhancement, relaxation, and reversal of the stereoselectivity for phosphotriesterase by rational evolution of active site residues.

2. Metabolix, Inc. and Tepha, Inc. Bioplastics for industry and medical devices.

3. Improving the catalytic activity of Candida antarctica lipase B by circular permutation.

4. Directed enzyme evolution via small and effective neutral drift libraries.

5. Engineering delta 9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor delta 9-18:0-ACP desaturase.

6. Improving catalytic function by ProSAR-driven enzyme evolution.

7. Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).

Review 8. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.

9. Selection and evolution of enzymes from a partially randomized non-catalytic scaffold.

10. Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution.

1. Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.

2. Crystallization and preliminary crystallographic analysis of LipC12, a true lipase isolated through a metagenomics approach.

3. Protein engineering: Check nature first, then evolve.

4. Rational assignment of key motifs for function guides in silico enzyme identification.

Review 5. Bacterial cocaine esterase: a protein-based therapy for cocaine overdose and addiction.

6. Biochemistry. Reengineering enzymes.

7. Protein engineering: Beating the odds.

8. Antibody humanization by structure-based computational protein design.

Review 9. Engineering the third wave of biocatalysis.

10. Fusion of an oligopeptide to the N terminus of an alkaline α-amylase from Alkalimonas amylolytica simultaneously improves the enzyme's catalytic efficiency, thermal stability, and resistance to oxidation.