Literature DB >> 20148555

RNA conformation in catalytically active human telomerase.

Justin A Yeoman1, Angel Orte, Beth Ashbridge, David Klenerman, Shankar Balasubramanian.   

Abstract

We have used single-molecule fluorescence microscopy to study the folded state of human telomerase RNA (hTR). Here we show that hTR adopts a new conformation on binding to human telomerase reverse transcriptase (hTERT) and reconstitution of an active ribonucleoprotein complex. Our data are consistent with the formation of an RNA pseudoknot in active human telomerase.

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Year:  2010        PMID： 20148555      PMCID： PMC3119468          DOI： 10.1021/ja909383n

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  15 in total

1.  Secondary structure of vertebrate telomerase RNA.

Authors:  J L Chen; M A Blasco; C W Greider
Journal:  Cell       Date:  2000-03-03       Impact factor: 41.582

2.  A role for a novel 'trans-pseudoknot' RNA-RNA interaction in the functional dimerization of human telomerase.

Authors:  Hinh Ly; Lifeng Xu; Melissa A Rivera; Tristram G Parslow; Elizabeth H Blackburn
Journal:  Genes Dev       Date:  2003-05-01       Impact factor: 11.361

3.  Functional analysis of the pseudoknot structure in human telomerase RNA.

Authors:  Jiunn-Liang Chen; Carol W Greider
Journal:  Proc Natl Acad Sci U S A       Date:  2005-04-22       Impact factor: 11.205

4.  The prediction of the wild-type telomerase RNA pseudoknot structure and the pivotal role of the bulge in its formation.

Authors:  Yaroslava G Yingling; Bruce A Shapiro
Journal:  J Mol Graph Model       Date:  2006-02-14       Impact factor: 2.518

5.  Fluorescence coincidence spectroscopy for single-molecule fluorescence resonance energy-transfer measurements.

Authors:  Angel Orte; Richard W Clarke; David Klenerman
Journal:  Anal Chem       Date:  2008-10-15       Impact factor: 6.986

6.  A molecular switch underlies a human telomerase disease.

Authors:  Luis R Comolli; Ivan Smirnov; Lifeng Xu; Elizabeth H Blackburn; Thomas L James
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-13       Impact factor: 11.205

7.  Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function.

Authors:  Carla A Theimer; Craig A Blois; Juli Feigon
Journal:  Mol Cell       Date:  2005-03-04       Impact factor: 17.970

8.  Solution structure and dynamics of the wild-type pseudoknot of human telomerase RNA.

Authors:  Nak-Kyoon Kim; Qi Zhang; Jing Zhou; Carla A Theimer; Robert D Peterson; Juli Feigon
Journal:  J Mol Biol       Date:  2008-10-11       Impact factor: 5.469

9.  Single-molecule analysis of human telomerase monomer.

Authors:  David Alves; Haitao Li; Rosalind Codrington; Angel Orte; Xiaojun Ren; David Klenerman; Shankar Balasubramanian
Journal:  Nat Chem Biol       Date:  2008-05       Impact factor: 15.040

10.  Structural analysis of the catalytic core of human telomerase RNA by FRET and molecular modeling.

Authors:  Gérald Gavory; Martyn F Symmons; Yamuna Krishnan Ghosh; David Klenerman; Shankar Balasubramanian
Journal:  Biochemistry       Date:  2006-11-07       Impact factor: 3.162
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  10 in total

1.  Functional importance of telomerase pseudoknot revealed by single-molecule analysis.

Authors:  Mariana Mihalusova; John Y Wu; Xiaowei Zhuang
Journal:  Proc Natl Acad Sci U S A       Date:  2011-05-13       Impact factor: 11.205

Review 2.  Unraveling secrets of telomeres: one molecule at a time.

Authors:  Jiangguo Lin; Parminder Kaur; Preston Countryman; Patricia L Opresko; Hong Wang
Journal:  DNA Repair (Amst)       Date:  2014-02-22

3.  An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity.

Authors:  Francesca S Gazzaniga; Elizabeth H Blackburn
Journal:  Blood       Date:  2014-10-15       Impact factor: 22.113

Review 4.  Single-molecule analysis of telomerase structure and function.

Authors:  Martin Hengesbach; Benjamin M Akiyama; Michael D Stone
Journal:  Curr Opin Chem Biol       Date:  2011-11-05       Impact factor: 8.822

5.  Single-molecule FRET reveals the folding dynamics of the human telomerase RNA pseudoknot domain.

Authors:  Martin Hengesbach; Nak-Kyoon Kim; Juli Feigon; Michael D Stone
Journal:  Angew Chem Int Ed Engl       Date:  2012-04-27       Impact factor: 15.336

6.  Single-molecule fluorescence resonance energy transfer studies of the human telomerase RNA pseudoknot: temperature-/urea-dependent folding kinetics and thermodynamics.

Authors:  Erik D Holmstrom; David J Nesbitt
Journal:  J Phys Chem B       Date:  2014-03-28       Impact factor: 2.991

7.  Refined secondary-structure models of the core of yeast and human telomerase RNAs directed by SHAPE.

Authors:  Rachel O Niederer; David C Zappulla
Journal:  RNA       Date:  2014-12-15       Impact factor: 4.942

8.  Human telomerase reverse transcriptase binds to a pre-organized hTR in vivo exposing its template.

Authors:  Georgeta Zemora; Stefan Handl; Christina Waldsich
Journal:  Nucleic Acids Res       Date:  2015-10-19       Impact factor: 16.971

Review 9.  Telomerase Regulation from Beginning to the End.

Authors:  Deanna Elise MacNeil; Hélène Jeanne Bensoussan; Chantal Autexier
Journal:  Genes (Basel)       Date:  2016-09-14       Impact factor: 4.096

10.  Different fluorophore labeling strategies and designs affect millisecond kinetics of DNA hairpins.

Authors:  Andreas Hartmann; Georg Krainer; Michael Schlierf
Journal:  Molecules       Date:  2014-09-03       Impact factor: 4.411
  10 in total

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