Literature DB >> 18391947

Single-molecule analysis of human telomerase monomer.

David Alves1, Haitao Li, Rosalind Codrington, Angel Orte, Xiaojun Ren, David Klenerman, Shankar Balasubramanian.   

Abstract

Human telomerase is a ribonucleoprotein that is minimally comprised of protein (hTERT) and RNA (hTR) components. We have applied single-molecule fluorescence two-color coincidence detection to characterize complex formation between fluorophore-labeled components in solution. By systematic labeling and in vitro assembly of hTERT, hTR and telomerase's DNA substrate, we have established that catalytically functional human telomerase comprises a stable hTERT:hTR:substrate interaction in a 1:1:1 absolute stoichiometry.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18391947     DOI： 10.1038/nchembio.82

Source DB:  PubMed          Journal:  Nat Chem Biol        ISSN： 1552-4450            Impact factor:   15.040


  24 in total

1.  Biological physics near surfaces/interfaces: a perspective.

Authors:  G Fragneto
Journal:  Eur Phys J E Soft Matter       Date:  2009-10-14       Impact factor: 1.890

2.  Single-molecule approach to immunoprecipitated protein complexes: insights into miRNA uridylation.

Authors:  Kyu-Hyeon Yeom; Inha Heo; Jinwoo Lee; Sungchul Hohng; V Narry Kim; Chirlmin Joo
Journal:  EMBO Rep       Date:  2011-07-01       Impact factor: 8.807

Review 3.  Unraveling secrets of telomeres: one molecule at a time.

Authors:  Jiangguo Lin; Parminder Kaur; Preston Countryman; Patricia L Opresko; Hong Wang
Journal:  DNA Repair (Amst)       Date:  2014-02-22

Review 4.  Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.

Authors:  Sujay Ray; Julia R Widom; Nils G Walter
Journal:  Chem Rev       Date:  2018-01-24       Impact factor: 60.622

Review 5.  Single-molecule analysis of telomerase structure and function.

Authors:  Martin Hengesbach; Benjamin M Akiyama; Michael D Stone
Journal:  Curr Opin Chem Biol       Date:  2011-11-05       Impact factor: 8.822

6.  Catalysis-dependent inactivation of human telomerase and its reactivation by intracellular telomerase-activating factors (iTAFs).

Authors:  Mohammed E Sayed; Ao Cheng; Gaya P Yadav; Andrew T Ludlow; Jerry W Shay; Woodring E Wright; Qiu-Xing Jiang
Journal:  J Biol Chem       Date:  2019-06-11       Impact factor: 5.157

7.  Rapid mapping of interactions between Human SNX-BAR proteins measured in vitro by AlphaScreen and single-molecule spectroscopy.

Authors:  Emma Sierecki; Loes M Stevers; Nichole Giles; Mark E Polinkovsky; Mehdi Moustaqil; Sergey Mureev; Wayne A Johnston; Mareike Dahmer-Heath; Dubravka Skalamera; Thomas J Gonda; Brian Gabrielli; Brett M Collins; Kirill Alexandrov; Yann Gambin
Journal:  Mol Cell Proteomics       Date:  2014-05-27       Impact factor: 5.911

8.  Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging.

Authors:  Roubina Tatavosian; Chao Yu Zhen; Huy Nguyen Duc; Maggie M Balas; Aaron M Johnson; Xiaojun Ren
Journal:  J Biol Chem       Date:  2015-09-17       Impact factor: 5.157

9.  A single-molecule assay for telomerase structure-function analysis.

Authors:  John Y Wu; Michael D Stone; Xiaowei Zhuang
Journal:  Nucleic Acids Res       Date:  2009-11-17       Impact factor: 16.971

10.  Single-molecule analysis of the human telomerase RNA.dyskerin interaction and the effect of dyskeratosis congenita mutations.

Authors:  Beth Ashbridge; Angel Orte; Justin A Yeoman; Michael Kirwan; Tom Vulliamy; Inderjeet Dokal; David Klenerman; Shankar Balasubramanian
Journal:  Biochemistry       Date:  2009-11-24       Impact factor: 3.162
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.