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Literature DB >> 2011524

A test case for physical mapping of human genome by repetitive sequence fingerprints: construction of a physical map of a 420 kb YAC subcloned into cosmids.

C Bellanné-Chantelot¹, E Barillot, B Lacroix, D Le Paslier, D Cohen.

Abstract

A rapid and safe method of Yeast Artificial Chromosome (YAC) physical mapping by cosmid 'fingerprinting' is presented. YACs are subcloned into cosmids which are prepared without previous separation of cloned DNA from host DNA. Groups of overlapping clones are detected according to their restriction fragments size and intensity after hybridization with total human DNA. To test this approach, a cosmid library was constructed from total DNA of a yeast strain containing a 420 kb YAC. A single contig of 84 clones was obtained with a minimal detectable overlap of 60% i.e. a 9.2 fold representative library. Large scale physical mapping of YACs would take full advantage of the DNA preparation procedure employed in this work and allows to take into account restriction fragment intensities.

Entities: Species

Mesh：

Year: 1991 PMID： 2011524 PMCID： PMC333640 DOI： 10.1093/nar/19.3.505

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. Toward a physical map of the genome of the nematode Caenorhabditis elegans.

Authors: A Coulson; J Sulston; S Brenner; J Karn
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

2. A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

Authors: R Anand; J H Riley; R Butler; J C Smith; A F Markham
Journal: Nucleic Acids Res Date: 1990-04-25 Impact factor: 16.971

3. Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

Authors: H M Albertsen; H Abderrahim; H M Cann; J Dausset; D Le Paslier; D Cohen
Journal: Proc Natl Acad Sci U S A Date: 1990-06 Impact factor: 11.205

A test case for physical mapping of human genome by repetitive sequence fingerprints: construction of a physical map of a 420 kb YAC subcloned into cosmids.

1. Toward a physical map of the genome of the nematode Caenorhabditis elegans.

2. A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

3. Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

4. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

5. The distribution of interspersed repetitive DNA sequences in the human genome.

6. Random-clone strategy for genomic restriction mapping in yeast.

7. A cosmid vector for systematic chromosome walking.

8. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

9. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

10. Physical mapping of complex genomes by cosmid multiplex analysis.

1. YAC subclone contig assembly by serial interspersed repetitive sequence (IRS)-PCR product hybridizations.