| Literature DB >> 20067781 |
Jong Kyu Lee1, Kyoung Sun Park, Seungil Park, Hyun Park, Young Hwan Song, Sung-Ho Kang, Hak Jun Kim.
Abstract
A psychrophilic yeast was isolated from an Arctic pond and its culture supernatant showed ice-binding activity. This isolate, identified as Leucosporidium sp. based on an analysis of the D1/D2 and ITS regions of its ribosomal DNA, produced a secretory ice-binding protein (IBP). Yeast IBP was purified from the culture medium to near homogeneity by the ice affinity method and appeared to be glycosylated with a molecular mass of approximately 26 kDa. In addition, the yeast IBP was shown to have thermal hysteresis (TH) and recrystallization inhibition (RI) activities. The full-length cDNA for yeast IBP was determined and was found to encode a 261 amino acid protein with molecular weight of 26.8 kDa that includes an N-terminal signal peptide and one potential N-glycosylation site. The deduced protein showed high sequence identity with other IBPs and hypothetical IBPs from fungi, diatoms, and bacteria, clustering with a class of ice-active proteins. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20067781 DOI： 10.1016/j.cryobiol.2010.01.002
Source DB: PubMed Journal: Cryobiology ISSN： 0011-2240 Impact factor: 2.487