Literature DB >> 32797415

Use of Ice Recrystallization Inhibition Assays to Screen for Compounds That Inhibit Ice Recrystallization.

Anna A Ampaw1, August Sibthorpe1, Robert N Ben2.   

Abstract

Ice recrystallization inhibition assays are used to screen for compounds that possess the ability to inhibit ice recrystallization. The most common of these assays are the splat cooling assay (SCA) and sucrose sandwich assay (SSA). These two assays possess similarities; however, they vary in their sample size, cooling rate, and the solution used to dissolve the analyte. In this chapter, both assay methods are described in detail, and we perform a direct comparison of the assays by evaluating the IRI activity of an antifreeze protein (AFP I). IRI activity is quantified by using ImageJ software to analyze ice crystals, and a quantitative value describing the efficiency of the inhibitor is generated. This analysis emphasizes the importance of choosing the right assay to measure IRI activity.

Entities:  

Keywords:  Antifreeze protein; Cryopreservation; Ice crystals; Ice recrystallization

Mesh：

Substances：

Year:  2021        PMID： 32797415     DOI： 10.1007/978-1-0716-0783-1_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  42 in total

1.  Prevention of freezing damage to living cells by dimethyl sulphoxide.

Authors:  J E LOVELOCK; M W BISHOP
Journal:  Nature       Date:  1959-05-16       Impact factor: 49.962

2.  The immobilization of spermatozoa by freezing and thawing and the protective action of glycerol.

Authors:  J E LOVELOCK; C POLGE
Journal:  Biochem J       Date:  1954-12       Impact factor: 3.857

3.  Ice recrystallization kinetics in the presence of synthetic antifreeze glycoprotein analogues using the framework of LSW theory.

Authors:  C Budke; C Heggemann; M Koch; N Sewald; T Koop
Journal:  J Phys Chem B       Date:  2009-03-05       Impact factor: 2.991

4.  C-linked antifreeze glycoprotein (C-AFGP) analogues as novel cryoprotectants.

Authors:  Mathieu Leclère; Bonnie K Kwok; Luke K Wu; David S Allan; Robert N Ben
Journal:  Bioconjug Chem       Date:  2011-08-04       Impact factor: 4.774

5.  Modulation of antifreeze activity and the effect upon post-thaw HepG2 cell viability after cryopreservation.

Authors:  Chantelle J Capicciotti; Jessica S Poisson; Christopher N Boddy; Robert N Ben
Journal:  Cryobiology       Date:  2015-01-13       Impact factor: 2.487

Review 6.  Stem Cells in the Treatment of Disease.

Authors:  Helen M Blau; George Q Daley
Journal:  N Engl J Med       Date:  2019-05-02       Impact factor: 91.245

7.  Differing actions of penetrating and nonpenetrating cryoprotective agents.

Authors:  L E McGann
Journal:  Cryobiology       Date:  1978-08       Impact factor: 2.487

8.  Small molecule ice recrystallization inhibitors enable freezing of human red blood cells with reduced glycerol concentrations.

Authors:  Chantelle J Capicciotti; Jayme D R Kurach; Tracey R Turner; Ross S Mancini; Jason P Acker; Robert N Ben
Journal:  Sci Rep       Date:  2015-04-08       Impact factor: 4.379

9.  Small-Molecule Ice Recrystallization Inhibitors Improve the Post-Thaw Function of Hematopoietic Stem and Progenitor Cells.

Authors:  Jennie G Briard; Suria Jahan; Priya Chandran; David Allan; Nicolas Pineault; Robert N Ben
Journal:  ACS Omega       Date:  2016-11-28

10.  Small molecule ice recrystallization inhibitors mitigate red blood cell lysis during freezing, transient warming and thawing.

Authors:  Jennie G Briard; Jessica S Poisson; Tracey R Turner; Chantelle J Capicciotti; Jason P Acker; Robert N Ben
Journal:  Sci Rep       Date:  2016-03-29       Impact factor: 4.379
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