Literature DB >> 20020744

Verification of biomarkers for diabetic retinopathy by multiple reaction monitoring.

Kyunggon Kim1, Sang Jin Kim, Hyeong Gon Yu, Jiyoung Yu, Kyong Soo Park, In-Jin Jang, Youngsoo Kim.   

Abstract

Multiple reaction monitoring was used to verify target proteins in 3 groups of vitreous and plasma samples from 3 stages of diabetic retinopathy: macular hole (nondiabetic control), nonproliferative diabetic retinopathy, and proliferative diabetic retinopathy. Twelve target proteins were quantified using triple quadrupole LC-MS/MS and 3 methods to determine the transitions (information-dependent analysis, the MIDAS workflow, and the PeptideAtlas database). This study might be the first MRM experiment to analyze large numbers of clinical vitreous and plasma samples for biomarker verification. Consequently, several biomarker candidates were identified for use in further applications.

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Year:  2010        PMID： 20020744     DOI： 10.1021/pr901013d

Source DB:  PubMed          Journal:  J Proteome Res        ISSN： 1535-3893            Impact factor:   4.466


  18 in total

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