Literature DB >> 20018187

The [FeFe]-hydrogenase maturase HydF from Clostridium acetobutylicum contains a CO and CN- ligated iron cofactor.

Ilka Czech1, Alexey Silakov, Wolfgang Lubitz, Thomas Happe.   

Abstract

Biosynthesis of the [FeFe] hydrogenases active site (H-cluster) requires three maturation factors whose respective roles are not understood yet. The clostridial maturation enzymes (CaHydE, CaHydF and CaHydG) were homologously overexpressed in their native host Clostridium acetobutylicum. CaHydF was able to activate Chlamydomonas reinhardtii [FeFe] hydrogenase apoprotein (CrHydA1(apo)) to almost 100% compared to the native specific hydrogen evolution activity. Based on electron paramagnetic resonance spectroscopy and Fourier-transform infrared spectroscopy data the existence of a [4Fe4S] cluster and a CO and CN(-) ligand coordinated di-iron cluster is suggested. This study contains the first experimental evidence that the bi-nuclear part of the H-cluster is assembled in HydF. 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Year:  2009        PMID： 20018187     DOI： 10.1016/j.febslet.2009.12.016

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  31 in total

1.  O2 reactions at the six-iron active site (H-cluster) in [FeFe]-hydrogenase.

Authors:  Camilla Lambertz; Nils Leidel; Kajsa G V Havelius; Jens Noth; Petko Chernev; Martin Winkler; Thomas Happe; Michael Haumann
Journal:  J Biol Chem       Date:  2011-09-19       Impact factor: 5.157

2.  Crystal structure of HydF scaffold protein provides insights into [FeFe]-hydrogenase maturation.

Authors:  Laura Cendron; Paola Berto; Sarah D'Adamo; Francesca Vallese; Chiara Govoni; Matthew C Posewitz; Giorgio M Giacometti; Paola Costantini; Giuseppe Zanotti
Journal:  J Biol Chem       Date:  2011-11-04       Impact factor: 5.157

Review 3.  Structure-function relationships in [FeFe]-hydrogenase active site maturation.

Authors:  Yvain Nicolet; Juan C Fontecilla-Camps
Journal:  J Biol Chem       Date:  2012-03-02       Impact factor: 5.157

4.  Evaluation of biosynthetic pathways for the unique dithiolate ligand of the FeFe hydrogenase H-cluster.

Authors:  Alexios Grigoropoulos; Robert K Szilagyi
Journal:  J Biol Inorg Chem       Date:  2010-09-10       Impact factor: 3.358

5.  The final steps of [FeFe]-hydrogenase maturation.

Authors:  Oliver Lampret; Julian Esselborn; Rieke Haas; Andreas Rutz; Rosalind L Booth; Leonie Kertess; Florian Wittkamp; Clare F Megarity; Fraser A Armstrong; Martin Winkler; Thomas Happe
Journal:  Proc Natl Acad Sci U S A       Date:  2019-07-23       Impact factor: 11.205

6.  Biomimetic assembly and activation of [FeFe]-hydrogenases.

Authors:  A Adamska; C Lambertz; T R Simmons; G Berggren; J Esselborn; M Atta; S Gambarelli; J M Mouesca; E Reijerse; W Lubitz; T Happe; V Artero; M Fontecave
Journal:  Nature       Date:  2013-06-26       Impact factor: 49.962

Review 7.  Radical S-adenosylmethionine enzymes.

Authors:  Joan B Broderick; Benjamin R Duffus; Kaitlin S Duschene; Eric M Shepard
Journal:  Chem Rev       Date:  2014-01-29       Impact factor: 60.622

8.  Nuclear resonance vibrational spectroscopy and electron paramagnetic resonance spectroscopy of 57Fe-enriched [FeFe] hydrogenase indicate stepwise assembly of the H-cluster.

Authors:  Jon M Kuchenreuther; Yisong Guo; Hongxin Wang; William K Myers; Simon J George; Christine A Boyke; Yoshitaka Yoda; E Ercan Alp; Jiyong Zhao; R David Britt; James R Swartz; Stephen P Cramer
Journal:  Biochemistry       Date:  2013-01-24       Impact factor: 3.162

9.  High-yield expression of heterologous [FeFe] hydrogenases in Escherichia coli.

Authors:  Jon M Kuchenreuther; Celestine S Grady-Smith; Alyssa S Bingham; Simon J George; Stephen P Cramer; James R Swartz
Journal:  PLoS One       Date:  2010-11-24       Impact factor: 3.240

10.  Cysteine as a ligand platform in the biosynthesis of the FeFe hydrogenase H cluster.

Authors:  Daniel L M Suess; Ingmar Bürstel; Liliana De La Paz; Jon M Kuchenreuther; Cindy C Pham; Stephen P Cramer; James R Swartz; R David Britt
Journal:  Proc Natl Acad Sci U S A       Date:  2015-08-31       Impact factor: 11.205
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