Literature DB >> 1999291

Cloning of trichosanthin cDNA and its expression in Escherichia coli.

P C Shaw1, M H Yung, R H Zhu, W K Ho, T B Ng, H W Yeung.   

Abstract

Several cDNA clones coding for trichosanthin (TCS) have been isolated from a cDNA library prepared from the poly(A)+RNA of the root tuber of Trichosanthes kirilowii Maximowicz. The nucleotide sequence codes for a protein of 289 amino acids (aa) including a putative signal peptide of 23 aa and an extra 19 aa at the C terminus; the latter two have not been found in TCS obtained from the natural product [Collins et al., J. Biol. Chem. 265 (1990) 8665-8669]. Recombinant TCS (reTCS) was synthesized in Escherichia coli, in which the cDNA without the signal sequence was expressed under the control of the trc promoter; reTCS was detected by a rabbit anti-TCS antiserum.

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Year:  1991        PMID： 1999291     DOI： 10.1016/0378-1119(91)90061-f

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  10 in total

1.  Momordin II, a ribosome inactivating protein from Momordica balsamina, is homologous to other plant proteins.

Authors:  M Ortigao; M Better
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

2.  BsiY I, a novel thermophilic restriction endonuclease that recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly sequenced site in pACYC177.

Authors:  Y K Mok; D R Clark; K M Kam; P C Shaw
Journal:  Nucleic Acids Res       Date:  1991-05-11       Impact factor: 16.971

3.  Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz.

Authors:  Hetian Lei; Jianjun Qi; Jingyuan Song; Dejun Yang; Yanzhi Wang; Yinling Zhang; Junshan Yang
Journal:  Plant Cell Rep       Date:  2006-06-07       Impact factor: 4.570

4.  Trichosanthin, a potent HIV-1 inhibitor, can cleave supercoiled DNA in vitro.

Authors:  M X Li; H W Yeung; L P Pan; S I Chan
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

Review 5.  Use of viral replicons for the expression of genes in plants.

Authors:  C Porta; G P Lomonossoff
Journal:  Mol Biotechnol       Date:  1996-06       Impact factor: 2.695

6.  Trichosanthin enhances anti-tumor immune response in a murine Lewis lung cancer model by boosting the interaction between TSLC1 and CRTAM.

Authors:  Yuchan Cai; Shudao Xiong; Yijie Zheng; Feifei Luo; Pei Jiang; Yiwei Chu
Journal:  Cell Mol Immunol       Date:  2011-05-16       Impact factor: 11.530

7.  Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.

Authors:  T Girbés; L Barbieri; M Ferreras; F J Arias; M A Rojo; R Iglesias; C Alegre; C Escarmis; F Stirpe
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

8.  Rapid, high-level expression of biologically active alpha-trichosanthin in transfected plants by an RNA viral vector.

Authors:  M H Kumagai; T H Turpen; N Weinzettl; G della-Cioppa; A M Turpen; J Donson; M E Hilf; G L Grantham; W O Dawson; T P Chow
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-15       Impact factor: 11.205

9.  Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors.

Authors:  J Zhao; L H Ben; Y L Wu; W Hu; K Ling; S M Xin; H L Nie; L Ma; G Pei
Journal:  J Exp Med       Date:  1999-07-05       Impact factor: 14.307

Review 10.  A Sixty-Year Research and Development of Trichosanthin, a Ribosome-Inactivating Protein.

Authors:  Jia-Qi Lu; Kam-Bo Wong; Pang-Chui Shaw
Journal:  Toxins (Basel)       Date:  2022-02-27       Impact factor: 4.546
  10 in total

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