Literature DB >> 1659689

Trichosanthin, a potent HIV-1 inhibitor, can cleave supercoiled DNA in vitro.

M X Li1, H W Yeung, L P Pan, S I Chan.   

Abstract

Trichosanthin, an abortifacient, immunosuppressive and anti-tumor protein purified from the traditional Chinese herb medicine Tian Hua Fen, is a potent inhibitor against HIV-1 replication. Under normal enzymatic digestion conditions, trichosanthin cleaves the supercoiled double-stranded DNA to produce nicked circular and linear DNA. Trichosanthin has no effect on linear double-stranded DNA. Neither does it convert relaxed circular duplex DNA into a supercoiled form in the presence of ATP. Thus trichosanthin is not a DNA gyrase. However, trichosanthin can cleave the relaxed circular DNA into a linear form, indicating that both the circular as well as the supercoiled forms are essential for trichosanthin recognition. In addition, trichosanthin contains one calcium metal ion per protein molecule, which presumably is related to its endonucleolytic activity.

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Year:  1991        PMID： 1659689      PMCID： PMC329144          DOI： 10.1093/nar/19.22.6309

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Modification of ribosomal RNA by ribosome-inactivating proteins from plants.

Authors:  F Stirpe; S Bailey; S P Miller; J W Bodley
Journal:  Nucleic Acids Res       Date:  1988-02-25       Impact factor: 16.971

2.  Primary amino acid sequence of alpha-trichosanthin and molecular models for abrin A-chain and alpha-trichosanthin.

Authors:  E J Collins; J D Robertus; M LoPresti; K L Stone; K R Williams; P Wu; K Hwang; M Piatak
Journal:  J Biol Chem       Date:  1990-05-25       Impact factor: 5.157

3.  Isolation and DNA sequence of a gene encoding alpha-trichosanthin, a type I ribosome-inactivating protein.

Authors:  T P Chow; R A Feldman; M Lovett; M Piatak
Journal:  J Biol Chem       Date:  1990-05-25       Impact factor: 5.157

4.  Purification and characterization of trichosanthin. Homology to the ricin A chain and implications as to mechanism of abortifacient activity.

Authors:  J M Maraganore; M Joseph; M C Bailey
Journal:  J Biol Chem       Date:  1987-08-25       Impact factor: 5.157

5.  Trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins.

Authors:  H W Yeung; W W Li; Z Feng; L Barbieri; F Stirpe
Journal:  Int J Pept Protein Res       Date:  1988-03

6.  The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

Authors:  U E Loening
Journal:  Biochem J       Date:  1967-01       Impact factor: 3.857

Review 7.  Plant and fungal protein and glycoprotein toxins inhibiting eukaryote protein synthesis.

Authors:  A Jiménez; D Vázquez
Journal:  Annu Rev Microbiol       Date:  1985       Impact factor: 15.500

8.  Isolation and characterization of an immunosuppressive protein from Trichosanthes kirilowii root tubers.

Authors:  H W Yeung; S P Poon; T B NG; W W Li
Journal:  Immunopharmacol Immunotoxicol       Date:  1987       Impact factor: 2.730

9.  Neural regulation of sex pheromone biosynthesis in Heliothis moths.

Authors:  P E Teal; J H Tumlinson; H Oberlander
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

10.  The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins.

Authors:  Y Endo; K Mitsui; M Motizuki; K Tsurugi
Journal:  J Biol Chem       Date:  1987-04-25       Impact factor: 5.157
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  20 in total

1.  The membrane insertion of trichosanthin is membrane-surface-pH dependent.

Authors:  X F Xia; S F Sui
Journal:  Biochem J       Date:  2000-08-01       Impact factor: 3.857

2.  Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz.

Authors:  Hetian Lei; Jianjun Qi; Jingyuan Song; Dejun Yang; Yanzhi Wang; Yinling Zhang; Junshan Yang
Journal:  Plant Cell Rep       Date:  2006-06-07       Impact factor: 4.570

3.  An additional mechanism of ribosome-inactivating protein cytotoxicity: degradation of extrachromosomal DNA.

Authors:  E Nicolas; I D Goodyer; T F Taraschi
Journal:  Biochem J       Date:  1997-10-15       Impact factor: 3.857

4.  Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: effect on DNA, RNA and poly(A).

Authors:  L Barbieri; P Valbonesi; E Bonora; P Gorini; A Bolognesi; F Stirpe
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

5.  Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells.

Authors:  C Zhang; Y Gong; H Ma; C An; D Chen; Z L Chen
Journal:  Biochem J       Date:  2001-05-01       Impact factor: 3.857

6.  A novel mechanism for inhibition of translation by pokeweed antiviral protein: depurination of the capped RNA template.

Authors:  K A Hudak; P Wang; N E Tumer
Journal:  RNA       Date:  2000-03       Impact factor: 4.942

7.  Functional Analysis of a Type-I Ribosome Inactivating Protein Balsamin from Momordica balsamina with Anti-Microbial and DNase Activity.

Authors:  Parminder Kaur Ajji; Ken Walder; Munish Puri
Journal:  Plant Foods Hum Nutr       Date:  2016-09       Impact factor: 3.921

Review 8.  The role of enzymatic activities of antiviral proteins from plants for action against plant pathogens.

Authors:  Nandlal Choudhary; M L Lodha; V K Baranwal
Journal:  3 Biotech       Date:  2020-11-04       Impact factor: 2.406

9.  Isolation and characterization of a novel ribosome-inactivating protein from root cultures of pokeweed and its mechanism of secretion from roots.

Authors:  Sang-Wook Park; Christopher B Lawrence; James C Linden; Jorge M Vivanco
Journal:  Plant Physiol       Date:  2002-09       Impact factor: 8.340

10.  Isolation and characterization of an RIP (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity.

Authors:  Neelam Sharma; Sang-Wook Park; Ramarao Vepachedu; Luigi Barbieri; Marialibera Ciani; Fiorenzo Stirpe; Brett J Savary; Jorge M Vivanco
Journal:  Plant Physiol       Date:  2003-12-11       Impact factor: 8.340
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