Ya-Ming Hou1, John J Perona. 1. Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th St., Philadelphia, PA 19107, USA. ya-ming.hou@jefferson.edu
Abstract
Methylation of tRNA on the four canonical bases adds structural complexity to the molecule, and improves decoding specificity and efficiency. While many tRNA methylases are known, detailed insight into the catalytic mechanism is only available in a few cases. Of interest among all tRNA methylases is the structural basis for nucleotide selection, by which the specificity is limited to a single site, or broadened to multiple sites. General themes in catalysis include the basis for rate acceleration at highly diverse nucleophilic centers for methyl transfer, using S-adenosylmethionine as a cofactor. Studies of tRNA methylases have also yielded insights into molecular evolution, particularly in the case of enzymes that recognize distinct structures to perform identical reactions at the same target nucleotide.
Methylation of tRNA on the four canonical bases adds structural complexity to the molecule, and improves decoding specificity and efficiency. While many tRNA methylases are known, detailed insight into the catalytic mechanism is only available in a few cases. Of interest among all tRNA methylases is the structural basis for nucleotide selection, by which the specificity is limited to a single site, or broadened to multiple sites. General themes in catan class="Chemical">lysis include the basis for rate acceleration at highly diverse nucleophilic centers for methyl transfer, using S-adenosylmethionine as a cofactor. Studies of tRNA methylases have also yielded insights into molecular evolution, particularly in the case of enzymes that recognize distinct structures to perform identical reactions at the same target nucleotide.
Authors: Martine Roovers; Johan Wouters; Janusz M Bujnicki; Catherine Tricot; Victor Stalon; Henri Grosjean; Louis Droogmans Journal: Nucleic Acids Res Date: 2004-01-22 Impact factor: 16.971
Authors: Jean Armengaud; Jaunius Urbonavicius; Bernard Fernandez; Guylaine Chaussinand; Janusz M Bujnicki; Henri Grosjean Journal: J Biol Chem Date: 2004-06-20 Impact factor: 5.157
Authors: Sujatha Kadaba; Anna Krueger; Tamyra Trice; Annette M Krecic; Alan G Hinnebusch; James Anderson Journal: Genes Dev Date: 2004-05-14 Impact factor: 11.361
Authors: Karen O'Dwyer; Joseph M Watts; Sanjoy Biswas; Jennifer Ambrad; Michael Barber; Hervé Brulé; Chantal Petit; David J Holmes; Magdalena Zalacain; Walter M Holmes Journal: J Bacteriol Date: 2004-04 Impact factor: 3.490
Authors: Sheena Menezes; Kirk W Gaston; Kady L Krivos; Ethel E Apolinario; Norbert O Reich; Kevin R Sowers; Patrick A Limbach; John J Perona Journal: Nucleic Acids Res Date: 2011-06-21 Impact factor: 16.971
Authors: Marcus Fislage; Martine Roovers; Irina Tuszynska; Janusz M Bujnicki; Louis Droogmans; Wim Versées Journal: Nucleic Acids Res Date: 2012-02-22 Impact factor: 16.971