Literature DB >> 1991728

Efficient transformation of Bacillus thuringiensis requires nonmethylated plasmid DNA.

A Macaluso1, A M Mettus.   

Abstract

The transformation efficiency of Bacillus thuringiensis depends upon the source of plasmid DNA. DNA isolated from B. thuringiensis, Bacillus megaterium, or a Dam- Dcm- Escherichia coli strain efficiently transformed several B. thuringiensis strains, B. thuringiensis strains were grouped according to which B. thuringiensis backgrounds were suitable sources of DNA for transformation of other B. thuringiensis strains, suggesting that B. thuringiensis strains differ in DNA modification and restriction. Efficient transformation allowed the demonstration of developmental regulation of cloned crystal protein genes in B. thuringiensis.

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Year:  1991        PMID： 1991728      PMCID： PMC207264          DOI： 10.1128/jb.173.3.1353-1356.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

1.  Location of DNA methylation genes on the Escherichia coli K-12 genetic map.

Authors:  M G Marinus
Journal:  Mol Gen Genet       Date:  1973-12-14

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Amino acid sequence and entomocidal activity of the P2 crystal protein. An insect toxin from Bacillus thuringiensis var. kurstaki.

Authors:  W P Donovan; C C Dankocsik; M P Gilbert; M C Gawron-Burke; R G Groat; B C Carlton
Journal:  J Biol Chem       Date:  1988-01-05       Impact factor: 5.157

4.  Transformation of Bacillus cereus vegetative cells by electroporation.

Authors:  B H Belliveau; J T Trevors
Journal:  Appl Environ Microbiol       Date:  1989-06       Impact factor: 4.792

5.  Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis.

Authors:  D J MacNeil
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

6.  Transformation of Bacillus thuringiensis by electroporation.

Authors:  E J Bone; D J Ellar
Journal:  FEMS Microbiol Lett       Date:  1989-04       Impact factor: 2.742

7.  Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis.

Authors:  D Lereclus; O Arantès; J Chaufaux; M Lecadet
Journal:  FEMS Microbiol Lett       Date:  1989-07-15       Impact factor: 2.742

8.  Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: phenotypic characterization of radiation-sensitive recombination-deficient mutants.

Authors:  D Dubnau; R Davidoff-Abelson; B Scher; C Cirigliano
Journal:  J Bacteriol       Date:  1973-04       Impact factor: 3.490

Review 9.  Insecticidal crystal proteins of Bacillus thuringiensis.

Authors:  H Höfte; H R Whiteley
Journal:  Microbiol Rev       Date:  1989-06

10.  Isolation and characterization of EG2158, a new strain of Bacillus thuringiensis toxic to coleopteran larvae, and nucleotide sequence of the toxin gene.

Authors:  W P Donovan; J M Gonzalez; M P Gilbert; C Dankocsik
Journal:  Mol Gen Genet       Date:  1988-11
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  31 in total

1.  Characterization of two genes encoding Bacillus thuringiensis insecticidal crystal proteins toxic to Coleoptera species.

Authors:  W P Donovan; M J Rupar; A C Slaney; T Malvar; M C Gawron-Burke; T B Johnson
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

Review 2.  Organization of restriction-modification systems.

Authors:  G G Wilson
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

3.  Expression of alkaline proteinase gene in two recombinant Bacillus cereus feather-degrading strains.

Authors:  H Ouled-Haddar; T I Zaghloul; H M Saeed
Journal:  Folia Microbiol (Praha)       Date:  2010-03-25       Impact factor: 2.099

4.  Development of a homologous expression system for and systematic site-directed mutagenesis analysis of thurincin H, a bacteriocin produced by Bacillus thuringiensis SF361.

Authors:  Gaoyan Wang; David C Manns; John J Churey; Randy W Worobo
Journal:  Appl Environ Microbiol       Date:  2014-06       Impact factor: 4.792

5.  Construction of Novel Bacillus thuringiensis Strains with Different Insecticidal Activities by Transduction and Transformation.

Authors:  M M Lecadet; J Chaufaux; J Ribier; D Lereclus
Journal:  Appl Environ Microbiol       Date:  1992-03       Impact factor: 4.792

Review 6.  The Bacillus cereus Group: Bacillus Species with Pathogenic Potential.

Authors:  Monika Ehling-Schulz; Didier Lereclus; Theresa M Koehler
Journal:  Microbiol Spectr       Date:  2019-05

7.  Cloning of the nprA gene for neutral protease A of Bacillus thuringiensis and effect of in vivo deletion of nprA on insecticidal crystal protein.

Authors:  W P Donovan; Y Tan; A C Slaney
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

Review 8.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

9.  Characterization and comparative sequence analysis of replication origins from three large Bacillus thuringiensis plasmids.

Authors:  J A Baum; M P Gilbert
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

10.  An engineered chymotrypsin/cathepsin G site in domain I renders Bacillus thuringiensis Cry3A active against Western corn rootworm larvae.

Authors:  Frederick S Walters; Cheryl M Stacy; Mi Kyong Lee; Narendra Palekar; Jeng S Chen
Journal:  Appl Environ Microbiol       Date:  2007-11-16       Impact factor: 4.792
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