Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Transformation of Bacillus cereus vegetative cells by electroporation.

Literature DB >> 2504113

Transformation of Bacillus cereus vegetative cells by electroporation.

Abstract

Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with unpurified total plasmid DNA.

Entities: Species

Mesh：

Substances：
DNA, Bacterial

Year: 1989 PMID： 2504113 PMCID： PMC202922 DOI： 10.1128/aem.55.6.1649-1652.1989

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

20 in total

1. Transfer of chromosomal genes and plasmids in Bacillus thuringiensis.

Authors: A I Aronson; W Beckman
Journal: Appl Environ Microbiol Date: 1987-07 Impact factor: 4.792

2. A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation.

Authors: Ian B Powell; Marc G Achen; Alan J Hillier; Barrie E Davidson
Journal: Appl Environ Microbiol Date: 1988-03 Impact factor: 4.792

Review 3. Bacillus thuringiensis and related insect pathogens.

Authors: A I Aronson; W Beckman; P Dunn
Journal: Microbiol Rev Date: 1986-03

4. Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells.

Authors: K Shigekawa; W J Dower
Journal: Biotechniques Date: 1988-09 Impact factor: 1.993

5. A substance produced by competent Bacillus cereus 569 cells that affects transformability.

Authors: I C Felkner; O Wyss
Journal: Biochem Biophys Res Commun Date: 1964-05-22 Impact factor: 3.575

6. Electroporation-induced transformation of intact cells of Clostridium perfringens.

Authors: S P Allen; H P Blaschek
Journal: Appl Environ Microbiol Date: 1988-09 Impact factor: 4.792

7. Enzymatic reduction of mercurous and mercuric ions in Bacillus cereus.

Authors: K Izaki
Journal: Can J Microbiol Date: 1981-02 Impact factor: 2.419

8. Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis.

Authors: J M González; B C Carlton
Journal: Plasmid Date: 1980-01 Impact factor: 3.466

9. Transformation of bacteria with plasmid DNA by electroporation.

Authors: S Fiedler; R Wirth
Journal: Anal Biochem Date: 1988-04 Impact factor: 3.365

10. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.

Authors: S D Ehrlich
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

9 in total

1. Evaluation of two transformation protocols and screening of positive plasmid introduction into Bacillus cereus EB2, a gram-positive bacterium using qualitative analyses.

Authors: Salwa Abdullah Sirajuddin; Shamala Sundram
Journal: Braz J Microbiol Date: 2020-02-20 Impact factor: 2.476

Transformation of Bacillus cereus vegetative cells by electroporation.

1. Transfer of chromosomal genes and plasmids in Bacillus thuringiensis.

2. A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation.

Review 3. Bacillus thuringiensis and related insect pathogens.

4. Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells.

5. A substance produced by competent Bacillus cereus 569 cells that affects transformability.

6. Electroporation-induced transformation of intact cells of Clostridium perfringens.

7. Enzymatic reduction of mercurous and mercuric ions in Bacillus cereus.

8. Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis.

9. Transformation of bacteria with plasmid DNA by electroporation.

10. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.

1. Evaluation of two transformation protocols and screening of positive plasmid introduction into Bacillus cereus EB2, a gram-positive bacterium using qualitative analyses.

2. Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus.

3. Mercury resistance determined by a self-transmissible plasmid in Bacillus cereus 5.

Review 4. Bacillus thuringiensis and its pesticidal crystal proteins.

Review 5. Gene transfer to plants by electroporation: methods and applications.

6. Electrotransformation of Thiobacillus ferrooxidans with plasmids containing a mer determinant.

7. Efficient transformation of Bacillus thuringiensis requires nonmethylated plasmid DNA.

8. Rapid protocol for preparation of electrocompetent Escherichia coli and Vibrio cholerae.

9. Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus.