Literature DB >> 198799

Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes.

E M Ross, A G Gilman.   

Abstract

Membranes of mouse L cells that contain adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] but lack beta-adrenergic receptors have been solubilized with Lubrol 12A9. Addition of such adenylate cyclase-containing extracts to beta-adrenergic receptor-replete membranes from adenylate cyclase-deficient S49 lymphoma cells results in the production of a catecholamine-sensitive adenylate cyclase system. The effects of beta-adrenergic agonists and antagonists on the reconstituted system reproduce those that are characteristic of the wild-type S49 lymphoma cell. The uncoupled variant of the S49lymphoma contains adenylate cyclase, but donor extracts from this clone fail to reconstitute the hormone-sensitive enzyme activity when added to adenylate cyclase-deficient membranes. Thus, the uncoupled and adenylate cyclase-deficient variants of the S49 cell are not complementary.

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Year:  1977        PMID： 198799      PMCID： PMC431700          DOI： 10.1073/pnas.74.9.3715

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Beta adrenergic receptors and adenylate cyclase: products of separate genes?

Authors:  P A Insel; M E Maguire; A G Gilman; H R Bourne; P Coffino; K L Melmon
Journal:  Mol Pharmacol       Date:  1976-11       Impact factor: 4.436

Review 3.  beta-Adrenergic receptor: ligand binding properties and the interaction with adenylyl cyclase.

Authors:  M E Maguire; E M Ross; A G Gilman
Journal:  Adv Cyclic Nucleotide Res       Date:  1977

4.  Formation of a novel nucleotide by fat cell preparations containing adenylate cyclase.

Authors:  B Petrack; D Ma; F Sheppy
Journal:  J Biol Chem       Date:  1974-06-10       Impact factor: 5.157

5.  Thyroid-stimulating hormone binding to beef thyroid membranes. Relation to adenylate cyclase activity.

Authors:  W V Moore; J Wolff
Journal:  J Biol Chem       Date:  1974-10-10       Impact factor: 5.157

6.  Adenylate cyclase permanently uncoupled from hormone receptors in a novel variant of S49 mouse lymphoma cells.

Authors:  T Haga; E M Ross; H J Anderson; A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

7.  Expression of genes for metabolism of cyclic adenosine 3':5'-monophosphate in somatic cells. beta-Adrenergic and PGE1 receptors in parental and hybrid cells.

Authors:  L L Brunton; M E Maguire; H J Anderson; A G Gilman
Journal:  J Biol Chem       Date:  1977-02-25       Impact factor: 5.157

8.  Resolution of beta-adrenergic receptor binding and adenylate cyclase activity by gel exclusion chromatography.

Authors:  L E Limbird; R J Lefkowitz
Journal:  J Biol Chem       Date:  1977-01-25       Impact factor: 5.157

9.  Catecholamine-stimulated GTPase activity in turkey erythrocyte membranes.

Authors:  D Cassel; Z Selinger
Journal:  Biochim Biophys Acta       Date:  1976-12-08

10.  Coupling of catecholamine receptor from one cell with adenylate cyclase from another cell by cell fusion.

Authors:  J Orly; M Schramm
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205
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  27 in total

1.  Coupling of opiate receptors to adenylate cyclase: requirement for Na+ and GTP.

Authors:  A J Blume; D Lichtshtein; G Boone
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

2.  Studies of cyclic AMP action using mutant tissue culture cells.

Authors:  P Coffino; H R Bourne; P A Insel; K L Melmon; G Johnson; J Vigne
Journal:  In Vitro       Date:  1978-01

Review 3.  The expanding roles and mechanisms of G protein-mediated presynaptic inhibition.

Authors:  Zack Zurawski; Yun Young Yim; Simon Alford; Heidi E Hamm
Journal:  J Biol Chem       Date:  2019-02-01       Impact factor: 5.157

4.  Inhibition of formation of cyclic AMP and cyclic GMP by vasopressin in smooth-muscle cells is insensitive to pertussis toxin.

Authors:  P Nambi; M Whitman; N Aiyar; S T Crooke
Journal:  Biochem J       Date:  1988-09-01       Impact factor: 3.857

5.  Reconstitution of cholera toxin-activated adenylate cyclase.

Authors:  G L Johnson; H R Kaslow; H R Bourne
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

6.  Properties of the interaction of fluoride- and guanylyl-5'-imidodiphosphate-regulatory proteins with adenylate cyclase.

Authors:  M Hebdon; H Le Vine; N Sahyoun; C J Schmitges; P Cuatrecasas
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

7.  Treatment of human platelets with trypsin, thrombin, or collagen inhibits the pertussis toxin-induced ADP-ribosylation of a 41-kDa protein.

Authors:  E G Lapetina; B Reep; K J Chang
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

8.  Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase.

Authors:  W A Toscano; K R Westcott; D C LaPorte; D R Storm
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

9.  Binding of agonists and antagonists to beta-adrenoceptors in rat vas deferens: relationship to functional response.

Authors:  J M May; P W Abel; K P Minneman
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1985-12       Impact factor: 3.000

10.  NHERF1 regulates parathyroid hormone receptor desensitization: interference with beta-arrestin binding.

Authors:  Bin Wang; Yanmei Yang; Abdul B Abou-Samra; Peter A Friedman
Journal:  Mol Pharmacol       Date:  2009-02-02       Impact factor: 4.436
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