Literature DB >> 293663

Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase.

W A Toscano, K R Westcott, D C LaPorte, D R Storm.   

Abstract

An adenylate cyclase [ATP pyrophosphatelyase (cyclizing), EC 4.6.1.1] preparation that is not stimulated by NaF,5'-guanylyl imidodiphosphate, or Ca2+.calmodulin has been isolated from bovine cerebral cortex by Affi-Gel Blue chromatography and calmodulin-Sepharose chromatography. Sensitivity to these effectors was restored by incubation of the adenylate cyclase preparation with detergent-solubilized protein from bovine cerebral cortex. Reconstitution of of Ca2+.calmodulin activation required the presence of 5'-guanylyl imidodiphosphate. The factor required for restoration of Ca2+.calmodulin stimulation was sensitive to heat, trypsin digestion, and N-ethylmaleimide. These observations suggest that this adenylate cyclase activity requires the presence of one or more guanyl nucleotide binding subunits for calmodulin sensitivity.

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Year:  1979        PMID： 293663      PMCID： PMC411693          DOI： 10.1073/pnas.76.11.5582

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Reconstitution of catecholamine-sensitive adenylate cyclase. Association of a regulatory component of the enzyme with membranes containing the catalytic protein and beta-adrenergic receptors.

Authors:  A C Howlett; P C Sternweis; B A Macik; P M Van Arsdale; A G Gilman
Journal:  J Biol Chem       Date:  1979-04-10       Impact factor: 5.157

2.  Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes.

Authors:  E M Ross; A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

3.  Complementation analysis of hormone-sensitive adenylate cyclase.

Authors:  J Naya-Vigne; G L Johnson; H R Bourne; P Coffino
Journal:  Nature       Date:  1978-04-20       Impact factor: 49.962

4.  Reconstitution of hormone-sensitive adenylate cyclase activity with resolved components of the enzyme.

Authors:  E M Ross; A C Howlett; K M Ferguson; A G Gilman
Journal:  J Biol Chem       Date:  1978-09-25       Impact factor: 5.157

5.  Reconstitution of cholera toxin-activated adenylate cyclase.

Authors:  G L Johnson; H R Kaslow; H R Bourne
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

6.  Properties of the interaction of fluoride- and guanylyl-5'-imidodiphosphate-regulatory proteins with adenylate cyclase.

Authors:  M Hebdon; H Le Vine; N Sahyoun; C J Schmitges; P Cuatrecasas
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

7.  Molecular resolution and reconstitution of the GPP (NH) P and NAF sensitive adenylate cyclase system.

Authors:  N Sahyoun; C J Schmitges; H Le Vine; P Cuatrecasas
Journal:  Life Sci       Date:  1977-12-15       Impact factor: 5.037

8.  GTP stimulates and inhibits adenylate cyclase in fat cell membranes through distinct regulatory processes.

Authors:  H Yamamura; P M Lad; M Rodbell
Journal:  J Biol Chem       Date:  1977-11-25       Impact factor: 5.157

9.  Reconstitution of catecholamine-sensitive adenylate cyclase. Reconstitution of the uncoupled variant of the S40 lymphoma cell.

Authors:  P C Sternweis; A G Gilman
Journal:  J Biol Chem       Date:  1979-05-10       Impact factor: 5.157

10.  Resolution of adenylate cyclase sensitive and insensitive to Ca2+ and calcium-dependent regulatory protein (CDR) by CDR-sepharose affinity chromatography.

Authors:  K R Westcott; D C La Porte; D R Storm
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205
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  5 in total

1.  Ca2+/calmodulin sensitivity may be common to all forms of neural adenylate cyclase.

Authors:  L S Eliot; Y Dudai; E R Kandel; T W Abrams
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

2.  Mechanism of action of choleragen and E. coli heat-labile enterotoxin: activation of adenylate cyclase by ADP-ribosylation.

Authors:  J Moss; M Vaughan
Journal:  Mol Cell Biochem       Date:  1981-07-07       Impact factor: 3.396

3.  GTP is not required for calmodulin stimulation of bovine brain adenylate cyclase.

Authors:  W Heideman; B M Wierman; D R Storm
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

4.  Properties of the separated catalytic and regulatory units of brain adenylate cyclase.

Authors:  S Strittmatter; E J Neer
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

5.  Differential effects of Ca2+-calmodulin on adenylate cyclase activity cyclase activity in mouse and rat pancreatic islets.

Authors:  P Thams; K Capito; C J Hedeskov
Journal:  Biochem J       Date:  1982-07-15       Impact factor: 3.857
  5 in total

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