Literature DB >> 3461464

Treatment of human platelets with trypsin, thrombin, or collagen inhibits the pertussis toxin-induced ADP-ribosylation of a 41-kDa protein.

E G Lapetina, B Reep, K J Chang.   

Abstract

Permeabilization of human platelets with saponin (15-25 micrograms/ml) allows the determination of the ADP-ribosylation of a 41-kDa protein by pertussis toxin. The ADP-ribosylated protein is present in the particulate fraction. ADP-ribosylation of the 41-kDa protein increases for 20 min; it is not affected by indomethacin, prostacyclin, and 1,2-diacylglycerols but is inhibited by 1 mM Ca2+ and phorbol esters. Treatment of platelets with trypsin, thrombin, or collagen before saponin addition precludes subsequent pertussis toxin-induced ADP-ribosylation of the 41-kDa protein. The effect of trypsin or thrombin is blocked by soybean trypsin inhibitor and leupeptin. Trypsin proteolytically cleaves the ADP-ribosylated 41-kDa protein to an ADP-ribosylated fragment slightly smaller than 20 kDa. The results suggest that a modification of a guanine nucleotide-binding regulatory protein is associated with the actions of trypsin, thrombin, and collagen on platelet activation.

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Year:  1986        PMID： 3461464      PMCID： PMC386400          DOI： 10.1073/pnas.83.16.5880

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  39 in total

1.  Pertussis toxin inhibition of chemotaxis and the ADP-ribosylation of a membrane protein in a human-mouse hybrid cell line.

Authors:  P S Backlund; B D Meade; C R Manclark; G L Cantoni; R R Aksamit
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

2.  Thrombin induces serotonin secretion and aggregation independently of inositol phospholipids hydrolysis and protein phosphorylation in human platelets permeabilized with saponin.

Authors:  E G Lapetina; J Silió; M Ruggiero
Journal:  J Biol Chem       Date:  1985-06-10       Impact factor: 5.157

3.  Leupeptin selectively inhibits human platelet responses induced by thrombin and trypsin; a role for proteolytic activation of phospholipase C.

Authors:  M Ruggiero; E G Lapetina
Journal:  Biochem Biophys Res Commun       Date:  1985-09-30       Impact factor: 3.575

4.  Agonist and antagonist binding to alpha 2-adrenergic receptors in purified membranes from human platelets. Implications of receptor-inhibitory nucleotide-binding protein stoichiometry.

Authors:  R R Neubig; R D Gantzos; R S Brasier
Journal:  Mol Pharmacol       Date:  1985-11       Impact factor: 4.436

5.  Receptor-mediated inhibition of adenylate cyclase and stimulation of arachidonic acid release in 3T3 fibroblasts. Selective susceptibility to islet-activating protein, pertussis toxin.

Authors:  T Murayama; M Ui
Journal:  J Biol Chem       Date:  1985-06-25       Impact factor: 5.157

6.  Pertussis toxin inhibits chemotactic peptide-stimulated generation of inositol phosphates and lysosomal enzyme secretion in human leukemic (HL-60) cells.

Authors:  S J Brandt; R W Dougherty; E G Lapetina; J E Niedel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

7.  Pertussis toxin inhibits fMet-Leu-Phe- but not phorbol ester-stimulated changes in rabbit neutrophils: role of G proteins in excitation response coupling.

Authors:  M Volpi; P H Naccache; T F Molski; J Shefcyk; C K Huang; M L Marsh; J Munoz; E L Becker; R I Sha'afi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

8.  ATP depletion in human platelets caused by permeabilization with saponin does not prevent serotonin secretion induced by collagen.

Authors:  M Ruggiero; T P Zimmerman; E G Lapetina
Journal:  Biochem Biophys Res Commun       Date:  1985-09-16       Impact factor: 3.575

9.  Guanosine 5'-O-thiotriphosphate stimulates phospholipase C activity in plasma membranes of rat hepatocytes.

Authors:  M A Wallace; J N Fain
Journal:  J Biol Chem       Date:  1985-08-15       Impact factor: 5.157

10.  Protease and cyclooxygenase inhibitors synergistically prevent activation of human platelets.

Authors:  M Ruggiero; E G Lapetina
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205
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  11 in total

1.  Inhibition of human platelet adenylate cyclase activity by adrenaline, thrombin and collagen: analysis and reinterpretation of experimental data.

Authors:  A Juska; R W Farndale
Journal:  Biochem J       Date:  1999-05-15       Impact factor: 3.857

2.  Inhibition of human platelet adenylate cyclase by collagen fibres. Effect of collagen is additive with that of adrenaline, but interactive with that of thrombin.

Authors:  R W Farndale; A B Winkler; B R Martin; M J Barnes
Journal:  Biochem J       Date:  1992-02-15       Impact factor: 3.857

3.  Subcellular distribution of alpha 2-adrenergic receptors, pertussis-toxin substrate and adenylate cyclase in human platelets.

Authors:  M A Zamorski; J C Ferraro; R R Neubig
Journal:  Biochem J       Date:  1990-02-01       Impact factor: 3.857

4.  Platelet aggregation induced by alpha 2-adrenoceptor and protein kinase C activation. A novel synergism.

Authors:  W Siess; E G Lapetina
Journal:  Biochem J       Date:  1989-10-15       Impact factor: 3.857

5.  Antibody specific to the alpha subunit of the guanine nucleotide-binding regulatory protein Go: developmental appearance and immunocytochemical localization in brain.

Authors:  K J Chang; W Pugh; S G Blanchard; J McDermed; J P Tam
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205

6.  Thrombolamban, the 22-kDa platelet substrate of cyclic AMP-dependent protein kinase, is immunologically homologous with the Ras family of GTP-binding proteins.

Authors:  T E White; J C Lacal; B Reep; T H Fischer; E G Lapetina; G C White
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

7.  Transforming growth factor beta 1 treatment of AKR-2B cells is coupled through a pertussis-toxin-sensitive G-protein(s).

Authors:  P H Howe; E B Leof
Journal:  Biochem J       Date:  1989-08-01       Impact factor: 3.857

8.  Epinephrine induces changes in the subcellular distribution of the inhibitory GTP-binding protein Gi alpha-2 and a 38-kDa phosphorylated protein in the human platelet.

Authors:  M F Crouch; D A Winegar; E G Lapetina
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

9.  Specific binding of [alpha-32P]GTP to cytosolic and membrane-bound proteins of human platelets correlates with the activation of phospholipase C.

Authors:  E G Lapetina; B R Reep
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

10.  Thrombin inhibits the pertussis-toxin-dependent ADP-ribosylation of a novel soluble Gi-protein in human platelets.

Authors:  J M Gennity; W Siess
Journal:  Biochem J       Date:  1991-11-01       Impact factor: 3.857
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