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Literature DB >> 1987027

Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli.

C J Phelps¹, D L Lyerly, J L Johnson, T D Wilkins.

Abstract

Cloned fragments constituting the 8.1-kb toxin A gene of Clostridium difficile were used to reconstruct the intact gene. The recombinant toxin expressed in Escherichia coli was cytotoxic, enterotoxic, and lethal. In addition, toxic lysate caused hemagglutination of rabbit erythrocytes. The toxic activities were inhibited by antibody specific for toxin A. Our findings demonstrate that the biological activities exhibited by native toxin A are functions of a single protein encoded by the 8.1-kb toxin A gene, independent of any other C. difficile gene products.

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Year: 1991 PMID： 1987027 PMCID： PMC257719 DOI： 10.1128/iai.59.1.150-153.1991

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

15 in total

1. Toxin A of Clostridium difficile is a potent cytotoxin.

Authors: K D Tucker; P E Carrig; T D Wilkins
Journal: J Clin Microbiol Date: 1990-05 Impact factor: 5.948

2. Effect of toxin A and B of Clostridium difficile on rabbit ileum and colon.

Authors: T J Mitchell; J M Ketley; S C Haslam; J Stephen; D W Burdon; D C Candy; R Daniel
Journal: Gut Date: 1986-01 Impact factor: 23.059

3. Biological activities of toxins A and B of Clostridium difficile.

Authors: D M Lyerly; D E Lockwood; S H Richardson; T D Wilkins
Journal: Infect Immun Date: 1982-03 Impact factor: 3.441

4. Purification and characterization of toxins A and B of Clostridium difficile.

Authors: N M Sullivan; S Pellett; T D Wilkins
Journal: Infect Immun Date: 1982-03 Impact factor: 3.441

5. Molecular cloning of Clostridium difficile toxin A gene fragment in lambda gt11.

Authors: L L Muldrow; G C Ibeanu; N I Lee; N K Bose; J Johnson
Journal: FEBS Lett Date: 1987-03-23 Impact factor: 4.124

6. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors: C Yanisch-Perron; J Vieira; J Messing
Journal: Gene Date: 1985 Impact factor: 3.688

7. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia.

Authors: J G Bartlett; T W Chang; M Gurwith; S L Gorbach; A B Onderdonk
Journal: N Engl J Med Date: 1978-03-09 Impact factor: 91.245

Review 8. Antibiotic-associated pseudomembranous colitis.

Authors: J G Bartlett
Journal: Rev Infect Dis Date: 1979 May-Jun

9. Enzyme-linked immunosorbent assay for Clostridium difficile toxin A.

Authors: D M Lyerly; N M Sullivan; T D Wilkins
Journal: J Clin Microbiol Date: 1983-01 Impact factor: 5.948

10. Effects of Clostridium difficile toxins given intragastrically to animals.

Authors: D M Lyerly; K E Saum; D K MacDonald; T D Wilkins
Journal: Infect Immun Date: 1985-02 Impact factor: 3.441

8 in total

1. Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A.

Authors: C Pothoulakis; R J Gilbert; C Cladaras; I Castagliuolo; G Semenza; Y Hitti; J S Montcrief; J Linevsky; C P Kelly; S Nikulasson; H P Desai; T D Wilkins; J T LaMont
Journal: J Clin Invest Date: 1996-08-01 Impact factor: 14.808

2. Comparative sequence analysis of the Clostridium difficile toxins A and B.

Authors: C von Eichel-Streiber; R Laufenberg-Feldmann; S Sartingen; J Schulze; M Sauerborn
Journal: Mol Gen Genet Date: 1992-05

Review 3. Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion.

Authors: C L Sears; J B Kaper
Journal: Microbiol Rev Date: 1996-03

4. Positive regulation of Clostridium difficile toxins.

Authors: J S Moncrief; L A Barroso; T D Wilkins
Journal: Infect Immun Date: 1997-03 Impact factor: 3.441

5. Clostridium difficile recombinant toxin A repeating units as a carrier protein for conjugate vaccines: studies of pneumococcal type 14, Escherichia coli K1, and Shigella flexneri type 2a polysaccharides in mice.

Authors: D Pavliakova; J S Moncrief; D M Lyerly; G Schiffman; D A Bryla; J B Robbins; R Schneerson
Journal: Infect Immun Date: 2000-04 Impact factor: 3.441