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Literature DB >> 1986373

Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.

P M Handley¹, M Mueckler, N R Siegel, A Ciechanover, A L Schwartz.

Abstract

The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1991 PMID： 1986373 PMCID： PMC50789 DOI： 10.1073/pnas.88.1.258

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

1. Primary structure of rat ceruloplasmin and analysis of tissue-specific gene expression during development.

Authors: R E Fleming; J D Gitlin
Journal: J Biol Chem Date: 1990-05-05 Impact factor: 5.157

2. Amino-terminal sequence identity of ubiquitin and the nonhistone component of nuclear protein A24.

Authors: L T Hunt; M O Dayhoff
Journal: Biochem Biophys Res Commun Date: 1977-01-24 Impact factor: 3.575

3. The mechanism of ubiquitin activating enzyme. A kinetic and equilibrium analysis.

Authors: A L Haas; I A Rose
Journal: J Biol Chem Date: 1982-09-10 Impact factor: 5.157

4. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

5. High-sensitivity sequencing with a gas-phase sequenator.

Authors: M W Hunkapiller; R M Hewick; W J Dreyer; L E Hood
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

6. Critical values for testing the significance of amino acid composition indexes.

Authors: A Cornish-Bowden
Journal: Anal Biochem Date: 1980-07-01 Impact factor: 3.365

7. Cloning of ubiquitin activating enzyme from wheat and expression of a functional protein in Escherichia coli.

Authors: P M Hatfield; J Callis; R D Vierstra
Journal: J Biol Chem Date: 1990-09-15 Impact factor: 5.157

8. Mutation of cysteine-88 in the Saccharomyces cerevisiae RAD6 protein abolishes its ubiquitin-conjugating activity and its various biological functions.

Authors: P Sung; S Prakash; L Prakash
Journal: Proc Natl Acad Sci U S A Date: 1990-04 Impact factor: 11.205

8. A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme.

Authors: K Kok; R Hofstra; A Pilz; A van den Berg; P Terpstra; C H Buys; B Carritt
Journal: Proc Natl Acad Sci U S A Date: 1993-07-01 Impact factor: 11.205

Review 9. Targeting the ubiquitin-mediated proteasome degradation of p53 for cancer therapy.

Authors: Tiffany Devine; Mu-Shui Dai
Journal: Curr Pharm Des Date: 2013 Impact factor: 3.116

Review 10. Protacs for treatment of cancer.

Authors: Kathleen M Sakamoto
Journal: Pediatr Res Date: 2010-05 Impact factor: 3.756

Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.

1. Primary structure of rat ceruloplasmin and analysis of tissue-specific gene expression during development.

2. Amino-terminal sequence identity of ubiquitin and the nonhistone component of nuclear protein A24.

3. The mechanism of ubiquitin activating enzyme. A kinetic and equilibrium analysis.

4. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

5. High-sensitivity sequencing with a gas-phase sequenator.

6. Critical values for testing the significance of amino acid composition indexes.

7. Cloning of ubiquitin activating enzyme from wheat and expression of a functional protein in Escherichia coli.

8. Mutation of cysteine-88 in the Saccharomyces cerevisiae RAD6 protein abolishes its ubiquitin-conjugating activity and its various biological functions.

9. DNA sequencing with chain-terminating inhibitors.

10. "Covalent affinity" purification of ubiquitin-activating enzyme.

Review 1. Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha.

Review 2. The role of human and mouse Y chromosome genes in male infertility.

3. Immunoelectron microscopic localization of the ubiquitin-activating enzyme E1 in HepG2 cells.

Review 4. [Ubiquitin-dependent degradation and modification of proteins].

5. Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.

Review 6. Ubiquitination involved enzymes and cancer.

7. A mouse Y chromosome pseudogene is related to human ubiquitin activating enzyme E1.

8. A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme.

Review 9. Targeting the ubiquitin-mediated proteasome degradation of p53 for cancer therapy.

Review 10. Protacs for treatment of cancer.