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Literature DB >> 1763053

Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.

Abstract

Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis.

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Year: 1991 PMID： 1763053 PMCID： PMC53140 DOI： 10.1073/pnas.88.24.11388

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

31 in total

1. A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1.

Authors: R G Kulka; B Raboy; R Schuster; H A Parag; G Diamond; A Ciechanover; M Marcus
Journal: J Biol Chem Date: 1988-10-25 Impact factor: 5.157

2. The gene coding a ubiquitin-activating enzyme may locate on X chromosome.

Authors: M Ohtsubo; T Nishimoto
Journal: Biochem Biophys Res Commun Date: 1988-06-30 Impact factor: 3.575

3. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors: W N Burnette
Journal: Anal Biochem Date: 1981-04 Impact factor: 3.365

Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.

1. A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1.

2. The gene coding a ubiquitin-activating enzyme may locate on X chromosome.

3. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

4. Metabolism of ubiquitinated histones.

5. Histone 2B can be modified by the attachment of ubiquitin.

6. Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85.

7. Intracellular localization of human DNA polymerase alpha with monoclonal antibodies.

8. The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzyme.

9. Intracellular localization of DNA polymerase alpha.

10. "Covalent affinity" purification of ubiquitin-activating enzyme.

1. Purification and characterization of a ubiquitin carrier protein kinase from HeLa cells.

2. Promoters active in interphase are bookmarked during mitosis by ubiquitination.