Literature DB >> 19837835

Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.

James Carroll1, Pierre Douarre, Aidan Coffey, Jim Buckley, Bill Cashman, Kevin O'Farrell, Jim O'Mahony.   

Abstract

A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.

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Year:  2009        PMID： 19837835      PMCID： PMC2794114          DOI： 10.1128/AEM.01203-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

Review 1.  Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine.

Authors:  N B Harris; R G Barletta
Journal:  Clin Microbiol Rev       Date:  2001-07       Impact factor: 26.132

2.  Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.

Authors:  P Kirschner; B Springer; U Vogel; A Meier; A Wrede; M Kiekenbeck; F C Bange; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-11       Impact factor: 5.948

3.  Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay.

Authors:  S G Franzblau; R S Witzig; J C McLaughlin; P Torres; G Madico; A Hernandez; M T Degnan; M B Cook; V K Quenzer; R M Ferguson; R H Gilman
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

4.  Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium.

Authors:  L Collins; S G Franzblau
Journal:  Antimicrob Agents Chemother       Date:  1997-05       Impact factor: 5.191

5.  Rapid screening of natural products for antimycobacterial activity by using luciferase-expressing strains of Mycobacterium bovis BCG and Mycobacterium intracellulare.

Authors:  R M Shawar; D J Humble; J M Van Dalfsen; C K Stover; M J Hickey; S Steele; L A Mitscher; W Baker
Journal:  Antimicrob Agents Chemother       Date:  1997-03       Impact factor: 5.191

Review 6.  Causation of Crohn's disease by Mycobacterium avium subspecies paratuberculosis.

Authors:  J Hermon-Taylor; T J Bull; J M Sheridan; J Cheng; M L Stellakis; N Sumar
Journal:  Can J Gastroenterol       Date:  2000-06       Impact factor: 3.522

7.  Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's disease.

Authors:  Tim J Bull; Elizabeth J McMinn; Karim Sidi-Boumedine; Angela Skull; Damien Durkin; Penny Neild; Glenn Rhodes; Roger Pickup; John Hermon-Taylor
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

8.  Differentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms.

Authors:  Makeda Semret; Christine Y Turenne; Petra de Haas; Desmond M Collins; Marcel A Behr
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

Review 9.  Contamination of food products with Mycobacterium avium paratuberculosis: a systematic review.

Authors:  M M Eltholth; V R Marsh; S Van Winden; F J Guitian
Journal:  J Appl Microbiol       Date:  2009-03-30       Impact factor: 3.772

10.  "In vitro" activities of antimycobacterial agents against Mycobacterium avium subsp. paratuberculosis linked to Crohn's disease and paratuberculosis.

Authors:  Stefania Zanetti; Paola Molicotti; Sara Cannas; Silvia Ortu; Niyaz Ahmed; Leonardo A Sechi
Journal:  Ann Clin Microbiol Antimicrob       Date:  2006-11-15       Impact factor: 3.944
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  4 in total

1.  'Nano-immuno test' for the detection of live Mycobacterium avium subspecies paratuberculosis bacilli in the milk samples using magnetic nano-particles and chromogen.

Authors:  Manju Singh; Shoor Vir Singh; Saurabh Gupta; Kundan Kumar Chaubey; Bjorn John Stephan; Jagdip Singh Sohal; Manali Dutta
Journal:  Vet Res Commun       Date:  2018-04-26       Impact factor: 2.459

2.  Gene encoded antimicrobial peptides, a template for the design of novel anti-mycobacterial drugs.

Authors:  James Carroll; Des Field; Paula M O'Connor; Paul D Cotter; Aidan Coffey; Colin Hill; R Paul Ross; Jim O'Mahony
Journal:  Bioeng Bugs       Date:  2010 Nov-Dec

3.  A Rapid Method for Quantifying Viable Mycobacterium avium subsp. paratuberculosis in Cellular Infection Assays.

Authors:  Hannah B Pooley; Kumudika de Silva; Auriol C Purdie; Douglas J Begg; Richard J Whittington; Karren M Plain
Journal:  Appl Environ Microbiol       Date:  2016-08-30       Impact factor: 4.792

4.  Investigating the spectrum of biological activity of ring-substituted salicylanilides and carbamoylphenylcarbamates.

Authors:  Jan Otevrel; Zuzana Mandelova; Matus Pesko; Jiahui Guo; Katarina Kralova; Frantisek Sersen; Marcela Vejsova; Danuta S Kalinowski; Zaklina Kovacevic; Aidan Coffey; Jozef Csollei; Des R Richardson; Josef Jampilek
Journal:  Molecules       Date:  2010-11-10       Impact factor: 4.411
  4 in total

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