Literature DB >> 19830594

The E. coli single protein production system for production and structural analysis of membrane proteins.

Lili Mao1, S Thangminlal Vaiphei, Tsutomu Shimazu, William M Schneider, Yuefeng Tang, Rajeswari Mani, Monica J Roth, Gaetano T Montelione, Masayori Inouye.   

Abstract

At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.

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Year:  2009        PMID: 19830594      PMCID: PMC4190415          DOI: 10.1007/s10969-009-9070-2

Source DB:  PubMed          Journal:  J Struct Funct Genomics        ISSN: 1345-711X


  10 in total

1.  Single protein production in living cells facilitated by an mRNA interferase.

Authors:  Motoo Suzuki; Junjie Zhang; Mohan Liu; Nancy A Woychik; Masayori Inouye
Journal:  Mol Cell       Date:  2005-04-15       Impact factor: 17.970

2.  Single protein production (SPP) system in Escherichia coli.

Authors:  Motoo Suzuki; Lili Mao; Masayori Inouye
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

3.  Staphylococcus aureus YoeB homologues inhibit translation initiation.

Authors:  Satoshi Yoshizumi; Yonglong Zhang; Yoshihiro Yamaguchi; Liang Chen; Barry N Kreiswirth; Masayori Inouye
Journal:  J Bacteriol       Date:  2009-07-06       Impact factor: 3.490

4.  Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms.

Authors:  E Wallin; G von Heijne
Journal:  Protein Sci       Date:  1998-04       Impact factor: 6.725

5.  Bacterial bioreactors for high yield production of recombinant protein.

Authors:  Motoo Suzuki; Rohini Roy; Haiyan Zheng; Nancy Woychik; Masayori Inouye
Journal:  J Biol Chem       Date:  2006-10-04       Impact factor: 5.157

6.  Characterization of mRNA interferases from Mycobacterium tuberculosis.

Authors:  Ling Zhu; Yonglong Zhang; Jiah-Shin Teh; Junjie Zhang; Nancy Connell; Harvey Rubin; Masayori Inouye
Journal:  J Biol Chem       Date:  2006-04-12       Impact factor: 5.157

Review 7.  mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems.

Authors:  Yoshihiro Yamaguchi; Masayori Inouye
Journal:  Prog Mol Biol Transl Sci       Date:  2009       Impact factor: 3.622

8.  Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system.

Authors:  Lili Mao; Yuefeng Tang; S Thangminlal Vaiphei; Tsutomu Shimazu; Sung-Gun Kim; Rajeswari Mani; Elias Fakhoury; Eileen White; Gaetano T Montelione; Masayori Inouye
Journal:  J Struct Funct Genomics       Date:  2009-10-24

9.  MazF, an mRNA interferase, mediates programmed cell death during multicellular Myxococcus development.

Authors:  Hirofumi Nariya; Masayori Inouye
Journal:  Cell       Date:  2008-01-11       Impact factor: 41.582

10.  MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli.

Authors:  Yonglong Zhang; Junjie Zhang; Klaus P Hoeflich; Mitsuhiko Ikura; Guoliang Qing; Masayori Inouye
Journal:  Mol Cell       Date:  2003-10       Impact factor: 17.970

  10 in total
  4 in total

1.  Suppression of phospholipid biosynthesis by cerulenin in the condensed Single-Protein-Production (cSPP) system.

Authors:  Lili Mao; Koichi Inoue; Yisong Tao; Gaetano T Montelione; Ann E McDermott; Masayori Inouye
Journal:  J Biomol NMR       Date:  2011-01-30       Impact factor: 2.835

Review 2.  Advances in protein NMR provided by the NIGMS Protein Structure Initiative: impact on drug discovery.

Authors:  Gaetano T Montelione; Thomas Szyperski
Journal:  Curr Opin Drug Discov Devel       Date:  2010-05

3.  Secretion of human superoxide dismutase in Escherichia coli using the condensed single-protein-production system.

Authors:  Lili Mao; Peter B Stathopulos; Mitsuhiko Ikura; Masayori Inouye
Journal:  Protein Sci       Date:  2010-12       Impact factor: 6.725

4.  High-throughput cloning and expression of integral membrane proteins in Escherichia coli.

Authors:  Renato Bruni; Brian Kloss
Journal:  Curr Protoc Protein Sci       Date:  2013-11-05
  4 in total

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