| Literature DB >> 19826084 |
Youichiro Wada1, Yoshihiro Ohta, Meng Xu, Shuichi Tsutsumi, Takashi Minami, Kenji Inoue, Daisuke Komura, Jun'ichi Kitakami, Nobuhiko Oshida, Argyris Papantonis, Akashi Izumi, Mika Kobayashi, Hiroko Meguro, Yasuharu Kanki, Imari Mimura, Kazuki Yamamoto, Chikage Mataki, Takao Hamakubo, Katsuhiko Shirahige, Hiroyuki Aburatani, Hiroshi Kimura, Tatsuhiko Kodama, Peter R Cook, Sigeo Ihara.
Abstract
Genome-wide studies reveal that transcription by RNA polymerase II (Pol II) is dynamically regulated. To obtain a comprehensive view of a single transcription cycle, we switched on transcription of five long human genes (>100 kbp) with tumor necrosis factor-alpha (TNFalpha) and monitored (using microarrays, RNA fluorescence in situ hybridization, and chromatin immunoprecipitation) the appearance of nascent RNA, changes in binding of Pol II and two insulators (the cohesin subunit RAD21 and the CCCTC-binding factor CTCF), and modifications of histone H3. Activation triggers a wave of transcription that sweeps along the genes at approximately 3.1 kbp/min; splicing occurs cotranscriptionally, a major checkpoint acts several kilobases downstream of the transcription start site to regulate polymerase transit, and Pol II tends to stall at cohesin/CTCF binding sites.Entities:
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Year: 2009 PMID： 19826084 PMCID： PMC2761237 DOI： 10.1073/pnas.0902573106
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205