| Literature DB >> 28405721 |
Arun Pandian Chandrasekaran1, Minjung Song2, Suresh Ramakrishna3,4.
Abstract
Human pluripotent stem cells comprise induced pluripotent and embryonic stem cells, which have tremendous potential for biological and therapeutic applications. The development of efficient technologies for the targeted genome alteration of stem cells in disease models is a prerequisite for utilizing stem cells to their full potential. Genome editing of stem cells is possible with the help of synthetic nucleases that facilitate site-specific modification of a gene of interest. Recent advances in genome editing techniques have improved the efficiency and speed of the development of stem cells for human disease models. Zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system are powerful tools for editing DNA at specific loci. Here, we discuss recent technological advances in genome editing with site-specific nucleases in human stem cells.Entities:
Keywords: Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system; Embryonic stem cells; Induced pluripotent stem cells; Transcription activator-like effector nucleases; Zinc finger nucleases
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Year: 2017 PMID： 28405721 DOI： 10.1007/s00018-017-2522-0
Source DB: PubMed Journal: Cell Mol Life Sci ISSN： 1420-682X Impact factor: 9.261