Literature DB >> 1979313

Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene.

M Navajas1, A M Laurent, J F Moreel, A Ragab, J P Cambou, G Cuny, F Cambien, G Roizès.   

Abstract

The apolipoprotein B gene is subject to mutations that may be important in coronary heart diseases. We have used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize a single nucleotide substitution in the apolipoprotein B gene. This mutation affects amino acid 4311 of the protein and converts asparagine to serine. It was found in 24% of the 81 unrelated individuals analyzed. Moreover, another mutation was detected by sequencing in a single individual.

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Year:  1990        PMID： 1979313     DOI： 10.1007/bf00205183

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  12 in total

1.  Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

Authors:  V C Sheffield; D R Cox; L S Lerman; R M Myers
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

2.  Use of denaturing gradient gel electrophoresis to detect point mutations in the factor VIII gene.

Authors:  M D Traystman; M Higuchi; C K Kasper; S E Antonarakis; H H Kazazian
Journal:  Genomics       Date:  1990-02       Impact factor: 5.736

3.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

4.  Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique.

Authors:  W W Noll; M Collins
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

5.  Common DNA polymorphism within coding sequence of apolipoprotein B gene associated with altered lipid levels.

Authors:  A Law; S C Wallis; L M Powell; R J Pease; H Brunt; L M Priestley; T J Knott; J Scott; D G Altman; G J Miller
Journal:  Lancet       Date:  1986-06-07       Impact factor: 79.321

6.  DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

Authors:  S G Fischer; L S Lerman
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

7.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

8.  DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

Authors:  S Tabor; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Structure of the human apolipoprotein B gene.

Authors:  B D Blackhart; E M Ludwig; V R Pierotti; L Caiati; M A Onasch; S C Wallis; L Powell; R Pease; T J Knott; M L Chu
Journal:  J Biol Chem       Date:  1986-11-25       Impact factor: 5.157
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  4 in total

1.  Analysis of the 5' flanking sequence of the G gamma globin gene by denaturing gradient gel electrophoresis confirms the heterogeneity of the Bantu beta S haplotype.

Authors:  G Tachdjian; M Benabdennebi; C Guidal; C Sayada; C Lapouméroulie; J Elion
Journal:  Hum Genet       Date:  1992 Sep-Oct       Impact factor: 4.132

2.  The polymorphism ApoB/4311 in patients with myocardial infarction and controls: the ECTIM Study.

Authors:  J F Moreel; G Roizes; A E Evans; D Arveiler; J P Cambou; C Souriau; H J Parra; E Desmarais; J C Fruchart; P Ducimetière
Journal:  Hum Genet       Date:  1992-05       Impact factor: 4.132

3.  Variant mapping of the Apo(B) AT rich minisatellite. Dependence on nucleotide sequence of the copy number variations. Instability of the non-canonical alleles.

Authors:  E Desmarais; S Vigneron; C Buresi; F Cambien; J P Cambou; G Roizes
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

4.  A very conservative region of ApoB-100 in the putative binding region to the LDL receptor in the Toulouse population.

Authors:  P Avoustin; H Mostachi; B Perret; J P Cambou; F Cambien; C de Préval
Journal:  Hum Genet       Date:  1992-12       Impact factor: 4.132
  4 in total

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