BACKGROUND: We conducted molecular analyses to confirm four clustering HIV-1 infections (Patient A, B, C & D) in Guangzhou, China. These cases were identified by epidemiological investigation and suspected to acquire the infection through a common heterosexual transmission chain. METHODS: Env C2V3V4 region, gag p17/p24 junction and partial pol gene of HIV-1 genome from serum specimens of these infected cases were amplified by reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequenced. RESULTS: Phylogenetic analyses indicated that their viral nucleotide sequences were significantly clustered together (bootstrap value is 99%, 98% and 100% in env, gag and pol tree respectively). Evolutionary distance analysis indicated that their genetic diversities of env, gag and pol genes were significantly lower than non-clustered controls, as measured by unpaired t-test (env gene comparison: p < 0.005; gag gene comparison: p < 0.005; pol gene comparison: p < 0.005). CONCLUSION: Epidemiological results and molecular analyses consistently illustrated these four cases represented a transmission chain which dispersed in the locality through heterosexual contact involving commercial sex worker.
BACKGROUND: We conducted molecular analyses to confirm four clustering HIV-1 infections (Patient A, B, C & D) in Guangzhou, China. These cases were identified by epidemiological investigation and suspected to acquire the infection through a common heterosexual transmission chain. METHODS:Env C2V3V4 region, gagp17/p24 junction and partial pol gene of HIV-1 genome from serum specimens of these infected cases were amplified by reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequenced. RESULTS: Phylogenetic analyses indicated that their viral nucleotide sequences were significantly clustered together (bootstrap value is 99%, 98% and 100% in env, gag and pol tree respectively). Evolutionary distance analysis indicated that their genetic diversities of env, gag and pol genes were significantly lower than non-clustered controls, as measured by unpaired t-test (env gene comparison: p < 0.005; gag gene comparison: p < 0.005; pol gene comparison: p < 0.005). CONCLUSION: Epidemiological results and molecular analyses consistently illustrated these four cases represented a transmission chain which dispersed in the locality through heterosexual contact involving commercial sex worker.
Authors: Michael L Metzker; David P Mindell; Xiao-Mei Liu; Roger G Ptak; Richard A Gibbs; David M Hillis Journal: Proc Natl Acad Sci U S A Date: 2002-10-18 Impact factor: 11.205
Authors: C P Goujon; V M Schneider; J Grofti; J Montigny; V Jeantils; P Astagneau; W Rozenbaum; F Lot; C Frocrain-Herchkovitch; N Delphin; F Le Gal; J C Nicolas; M C Milinkovitch; P Dény Journal: J Virol Date: 2000-03 Impact factor: 5.103
Authors: C Y Ou; C A Ciesielski; G Myers; C I Bandea; C C Luo; B T Korber; J I Mullins; G Schochetman; R L Berkelman; A N Economou Journal: Science Date: 1992-05-22 Impact factor: 47.728
Authors: Tommy W C Leung; Darwin Mak; K H Wong; Y Wang; Y H Song; D N C Tsang; C Wong; Y M Shao; W L Lim Journal: AIDS Res Hum Retroviruses Date: 2008-07 Impact factor: 2.205
Authors: T J Palker; M E Clark; A J Langlois; T J Matthews; K J Weinhold; R R Randall; D P Bolognesi; B F Haynes Journal: Proc Natl Acad Sci U S A Date: 1988-03 Impact factor: 11.205
Authors: J R Rusche; K Javaherian; C McDanal; J Petro; D L Lynn; R Grimaila; A Langlois; R C Gallo; L O Arthur; P J Fischinger Journal: Proc Natl Acad Sci U S A Date: 1988-05 Impact factor: 11.205
Authors: Uma Shanmugasundaram; Suniti Solomon; Kailapuri G Murugavel; Kumarasamy Nagalingeswaran; Sunil S Solomon; Kenneth H Mayer; Balakrishnan Pachamuthu Journal: AIDS Res Hum Retroviruses Date: 2010-08 Impact factor: 2.205
Authors: August Guang; Mark Howison; Lauren Ledingham; Matthew D'Antuono; Philip A Chan; Charles Lawrence; Casey W Dunn; Rami Kantor Journal: Front Microbiol Date: 2022-02-17 Impact factor: 6.064
Authors: Vladimir Novitsky; Hermann Bussmann; Andrew Logan; Sikhulile Moyo; Erik van Widenfelt; Lillian Okui; Mompati Mmalane; Jeannie Baca; Lauren Buck; Eleanor Phillips; David Tim; Mary Fran McLane; Quanhong Lei; Rui Wang; Joseph Makhema; Shahin Lockman; Victor DeGruttola; M Essex Journal: PLoS One Date: 2013-12-11 Impact factor: 3.240