BACKGROUND: Phylogenetic and genetic analyses have proven a valuable tool to infer epidemiological links between human immunodeficiency virus type-1 (HIV-1) isolates. These methods were applied in the present report for studying the genetic relatedness of the viral strains involved in two episodes of suspected HIV-1 transmission. OBJECTIVES: Provide any evidence that may help establish or refute the transmission link. STUDY DESIGN: In the first case, a leukemic patient became HIV-1 positive following the transfusion of platelets from a donor who was subsequently found to have tested false HIV-seronegative and to be sexual partner to an infected woman. In the second, a wife claimed to have acquired the infection from her husband who had concealed his infected status. RESULTS AND CONCLUSIONS: The viral pairs detected in each of the suspected transmission cases exhibited common amino acid signatures and low genetic distances and segregated together in phylogenetic trees, thus showing a level of genetic relatedness similar to reference pairs known with certainty to be epidemiologically linked. These findings corroborated the existence of a direct transmission link in both the episodes with a high level of confidence.
BACKGROUND: Phylogenetic and genetic analyses have proven a valuable tool to infer epidemiological links between human immunodeficiency virus type-1 (HIV-1) isolates. These methods were applied in the present report for studying the genetic relatedness of the viral strains involved in two episodes of suspected HIV-1 transmission. OBJECTIVES: Provide any evidence that may help establish or refute the transmission link. STUDY DESIGN: In the first case, a leukemicpatient became HIV-1 positive following the transfusion of platelets from a donor who was subsequently found to have tested false HIV-seronegative and to be sexual partner to an infected woman. In the second, a wife claimed to have acquired the infection from her husband who had concealed his infected status. RESULTS AND CONCLUSIONS: The viral pairs detected in each of the suspected transmission cases exhibited common amino acid signatures and low genetic distances and segregated together in phylogenetic trees, thus showing a level of genetic relatedness similar to reference pairs known with certainty to be epidemiologically linked. These findings corroborated the existence of a direct transmission link in both the episodes with a high level of confidence.
Authors: Austin L Hughes; Shelby O'Connor; Dawn M Dudley; Benjamin J Burwitz; Benjamin N Bimber; David O'Connor Journal: Infect Genet Evol Date: 2010-02-10 Impact factor: 3.342
Authors: Zhigang Han; Tommy W C Leung; Jinkou Zhao; Ming Wang; Lirui Fan; Kai Li; Xinli Pang; Zhenbo Liang; Wilina W L Lim; Huifang Xu Journal: Virol J Date: 2009-09-25 Impact factor: 4.099
Authors: Ann M Dennis; Joshua T Herbeck; Andrew L Brown; Paul Kellam; Tulio de Oliveira; Deenan Pillay; Christophe Fraser; Myron S Cohen Journal: J Acquir Immune Defic Syndr Date: 2014-10-01 Impact factor: 3.731
Authors: Gonzalo Moratorio; Mariela Martínez; María F Gutiérrez; Katiuska González; Rodney Colina; Fernando López-Tort; Lilia López; Ricardo Recarey; Alejandro G Schijman; María P Moreno; Laura García-Aguirre; Aura R Manascero; Juan Cristina Journal: Virol J Date: 2007-08-02 Impact factor: 4.099