Literature DB >> 19722611

Reverse pH-dependence of chromophore protonation explains the large Stokes shift of the red fluorescent protein mKeima.

Sebastien Violot1, Philippe Carpentier, Laurent Blanchoin, Dominique Bourgeois.   

Abstract

The recently developed red fluorescent protein Keima exhibits the largest Stokes shift (180 nm) observed to date. Combining X-ray crystallography with (in crystallo) UV-visible absorption, fluorescence, and Raman spectroscopy, we have investigated molecular determinants of this peculiar property. The results demonstrate a pH-dependent "reverse chromophore protonation" triggered by the key residue Asp157 and which couples to cis/trans isomerization of the chromophore. These data provided guidelines to rationally design a useful Keima variant.

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Year:  2009        PMID： 19722611     DOI： 10.1021/ja903695n

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  29 in total

1.  Imaging intracellular pH in live cells with a genetically encoded red fluorescent protein sensor.

Authors:  Mathew Tantama; Yin Pun Hung; Gary Yellen
Journal:  J Am Chem Soc       Date:  2011-06-09       Impact factor: 15.419

2.  Monitoring Autophagy by Optical Microscopy.

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3.  Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large Stokes shift.

Authors:  Kiryl D Piatkevich; Vladimir N Malashkevich; Steven C Almo; Vladislav V Verkhusha
Journal:  J Am Chem Soc       Date:  2010-08-11       Impact factor: 15.419

4.  Monomeric red fluorescent proteins with a large Stokes shift.

Authors:  Kiryl D Piatkevich; James Hulit; Oksana M Subach; Bin Wu; Arian Abdulla; Jeffrey E Segall; Vladislav V Verkhusha
Journal:  Proc Natl Acad Sci U S A       Date:  2010-03-08       Impact factor: 11.205

5.  Light-activated reassembly of split green fluorescent protein.

Authors:  Kevin P Kent; Steven G Boxer
Journal:  J Am Chem Soc       Date:  2011-02-25       Impact factor: 15.419

6.  Nanopatterned Protein Films Directed by Ionic Complexation with Water-Soluble Diblock Copolymers.

Authors:  Bokyung Kim; Christopher N Lam; Bradley D Olsen
Journal:  Macromolecules       Date:  2012-06-12       Impact factor: 5.985

7.  Spectral and structural analysis of large Stokes shift fluorescent protein dKeima570.

Authors:  Yongbin Xu; Kwang Yeon Hwang; Ki Hyun Nam
Journal:  J Microbiol       Date:  2018-10-24       Impact factor: 3.422

8.  Structural Consequences of Chromophore Formation and Exploration of Conserved Lid Residues amongst Naturally Occurring Fluorescent Proteins.

Authors:  Matthew H Zimmer; Binsen Li; Ramza S Shahid; Paola Peshkepija; Marc Zimmer
Journal:  Chem Phys       Date:  2014-01-31       Impact factor: 2.348

9.  Excited state proton transfer in the red fluorescent protein mKeima.

Authors:  J Nathan Henderson; Maire F Osborn; Nayden Koon; Rinat Gepshtein; Dan Huppert; S James Remington
Journal:  J Am Chem Soc       Date:  2009-09-23       Impact factor: 15.419

Review 10.  Photoswitchable fluorescent proteins: ten years of colorful chemistry and exciting applications.

Authors:  Xin X Zhou; Michael Z Lin
Journal:  Curr Opin Chem Biol       Date:  2013-07-19       Impact factor: 8.822
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