Literature DB >> 1971945

DNA binding properties of the purified Antennapedia homeodomain.

M Affolter1, A Percival-Smith, M Müller, W Leupin, W J Gehring.   

Abstract

The in vitro DNA binding properties of a purified 68-amino acid Antennapedia homeodomain (Antp HD) peptide have been analyzed. Equilibrium and kinetic binding studies showed that stable DNA-protein complexes are formed with a Kd of 1.6 x 10(-9) M and 1.8 x 10(-10) M, respectively. Heterodimer analysis led to the conclusion that Antp HD interacts in vitro as a monomer with the DNA target sites used in our study. The results of methylation and ethylation interference studies indicated that the Antp HD closely approaches the target DNA primarily from one side in a region extending across three phosphate backbones. The DNA binding properties of the Antp HD and prokaryotic DNA binding domains that share a helix-turn-helix motif are compared.

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Year:  1990        PMID： 1971945      PMCID： PMC54053          DOI： 10.1073/pnas.87.11.4093

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

1.  The structure of the Antennapedia homeodomain determined by NMR spectroscopy in solution: comparison with prokaryotic repressors.

Authors:  Y Q Qian; M Billeter; G Otting; M Müller; W J Gehring; K Wüthrich
Journal:  Cell       Date:  1989-11-03       Impact factor: 41.582

2.  A single amino acid can determine the DNA binding specificity of homeodomain proteins.

Authors:  J Treisman; P Gönczy; M Vashishtha; E Harris; C Desplan
Journal:  Cell       Date:  1989-11-03       Impact factor: 41.582

3.  DNA specificity of the bicoid activator protein is determined by homeodomain recognition helix residue 9.

Authors:  S D Hanes; R Brent
Journal:  Cell       Date:  1989-06-30       Impact factor: 41.582

4.  Structure of a phage 434 Cro/DNA complex.

Authors:  C Wolberger; Y C Dong; M Ptashne; S C Harrison
Journal:  Nature       Date:  1988-10-27       Impact factor: 49.962

5.  Kinetic studies on Cro repressor-operator DNA interaction.

Authors:  J G Kim; Y Takeda; B W Matthews; W F Anderson
Journal:  J Mol Biol       Date:  1987-07-05       Impact factor: 5.469

6.  The operator-binding domain of lambda repressor: structure and DNA recognition.

Authors:  C O Pabo; M Lewis
Journal:  Nature       Date:  1982-07-29       Impact factor: 49.962

7.  Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.

Authors:  U Siebenlist; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

8.  Changing the binding specificity of a repressor by redesigning an alpha-helix.

Authors:  R P Wharton; M Ptashne
Journal:  Nature       Date:  1985 Aug 15-21       Impact factor: 49.962

9.  Flexibility of the DNA-binding domains of trp repressor.

Authors:  C L Lawson; R G Zhang; R W Schevitz; Z Otwinowski; A Joachimiak; P B Sigler
Journal:  Proteins       Date:  1988

10.  Structural organization and sequence of the homeotic gene Antennapedia of Drosophila melanogaster.

Authors:  S Schneuwly; A Kuroiwa; P Baumgartner; W J Gehring
Journal:  EMBO J       Date:  1986-04       Impact factor: 11.598
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  81 in total

1.  Expression and function of the homeodomain-containing protein Hex in thyroid cells.

Authors:  L Pellizzari; A D'Elia; A Rustighi; G Manfioletti; G Tell; G Damante
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

2.  Characterization of Hoxd1 protein-DNA-binding specificity using affinity chromatography and random DNA oligomer selection.

Authors:  P Kumar; A J Nazarali
Journal:  Cell Mol Neurobiol       Date:  2001-08       Impact factor: 5.046

3.  Regulation by homeoproteins: a comparison of deformed-responsive elements.

Authors:  J A Pederson; J W LaFollette; C Gross; A Veraksa; W McGinnis; J W Mahaffey
Journal:  Genetics       Date:  2000-10       Impact factor: 4.562

4.  DNA topology, not DNA sequence, is a critical determinant for Drosophila ORC-DNA binding.

Authors:  Dirk Remus; Eileen L Beall; Michael R Botchan
Journal:  EMBO J       Date:  2004-02-05       Impact factor: 11.598

5.  Differential DNA binding properties of three human homeodomain proteins.

Authors:  M T Corsetti; P Briata; L Sanseverino; A Daga; I Airoldi; A Simeone; G Palmisano; C Angelini; E Boncinelli; G Corte
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

6.  Sequence-specific DNA binding by the vnd/NK-2 homeodomain of Drosophila.

Authors:  Lan-Hsiang Wang; Rebecca Chmelik; Marshall Nirenberg
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-13       Impact factor: 11.205

7.  Sequence-specific DNA binding by the MspI DNA methyltransferase.

Authors:  A K Dubey; R J Roberts
Journal:  Nucleic Acids Res       Date:  1992-06-25       Impact factor: 16.971

8.  Several regions of Antennapedia and thyroid transcription factor 1 homeodomains contribute to DNA binding specificity.

Authors:  G Damante; R Di Lauro
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

9.  NMR structural and kinetic characterization of a homeodomain diffusing and hopping on nonspecific DNA.

Authors:  Junji Iwahara; Markus Zweckstetter; G Marius Clore
Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-28       Impact factor: 11.205

10.  Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites.

Authors:  P A Beachy; J Varkey; K E Young; D P von Kessler; B I Sun; S C Ekker
Journal:  Mol Cell Biol       Date:  1993-11       Impact factor: 4.272
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