Literature DB >> 19699703

Quantification of extracellular UDP-galactose.

Eduardo R Lazarowski1.   

Abstract

The human P2Y(14) receptor is potently activated by UDP-glucose (UDP-Glc), UDP-galactose (UDP-Gal), UDP-N-acetylglucosamine (UDP-GlcNAc), and UDP-glucuronic acid. Recently, cellular release of UDP-Glc and UDP-GlcNAc has been reported, but whether additional UDP-sugars are endogenous agonists for the P2Y(14) receptor remains poorly defined. In the present study, we describe an assay for the quantification of UDP-Gal with subnanomolar sensitivity. This assay is based on the enzymatic conversion of UDP-Gal to UDP, using 1-4-beta-galactosyltransferase. UDP is subsequently phosphorylated by nucleoside diphosphokinase in the presence of [gamma-(32)P]ATP and the formation of [gamma-(32)P]UTP is monitored by high-performance liquid chromatography. The overall conversion of UDP-Gal to [gamma-(32)P]UTP was linear between 0.5 and 30 nM UDP-Gal. Extracellular UDP-Gal was detected on resting cultures of various cell types, and increased release of UDP-Gal was observed in 1321N1 human astrocytoma cells stimulated with the protease-activated receptor agonist thrombin. The occurrence of regulated release of UDP-Gal suggests that, in addition to its role in glycosylation reactions, UDP-Gal is an important extracellular signaling molecule.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19699703      PMCID： PMC2790022          DOI： 10.1016/j.ab.2009.08.022

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  36 in total

Review 1.  Reflections on glycobiology.

Authors:  S Roseman
Journal:  J Biol Chem       Date:  2001-09-11       Impact factor: 5.157

2.  Nucleotide-mediated calcium signaling in rat cortical astrocytes: Role of P2X and P2Y receptors.

Authors:  Marta Fumagalli; Roberta Brambilla; Nadia D'Ambrosi; Cinzia Volonté; Michela Matteoli; Claudia Verderio; Maria P Abbracchio
Journal:  Glia       Date:  2003-09       Impact factor: 7.452

Review 3.  Mechanisms of release of nucleotides and integration of their action as P2X- and P2Y-receptor activating molecules.

Authors:  Eduardo R Lazarowski; Richard C Boucher; T Kendall Harden
Journal:  Mol Pharmacol       Date:  2003-10       Impact factor: 4.436

4.  Intracellular nucleotide and nucleotide sugar contents of cultured CHO cells determined by a fast, sensitive, and high-resolution ion-pair RP-HPLC.

Authors:  N Kochanowski; F Blanchard; R Cacan; F Chirat; E Guedon; A Marc; J-L Goergen
Journal:  Anal Biochem       Date:  2005-11-15       Impact factor: 3.365

5.  A G protein-coupled receptor for UDP-glucose.

Authors:  J K Chambers; L E Macdonald; H M Sarau; R S Ames; K Freeman; J J Foley; Y Zhu; M M McLaughlin; P Murdock; L McMillan; J Trill; A Swift; N Aiyar; P Taylor; L Vawter; S Naheed; P Szekeres; G Hervieu; C Scott; J M Watson; A J Murphy; E Duzic; C Klein; D J Bergsma; S Wilson; G P Livi
Journal:  J Biol Chem       Date:  2000-04-14       Impact factor: 5.157

6.  Thrombin-promoted release of UDP-glucose from human astrocytoma cells.

Authors:  S M Kreda; L Seminario-Vidal; C van Heusden; E R Lazarowski
Journal:  Br J Pharmacol       Date:  2008-01-21       Impact factor: 8.739

7.  GPR105, a novel Gi/o-coupled UDP-glucose receptor expressed on brain glia and peripheral immune cells, is regulated by immunologic challenge: possible role in neuroimmune function.

Authors:  Darren J Moore; Paul R Murdock; Jeannette M Watson; Richard L M Faull; Henry J Waldvogel; Philip G Szekeres; Shelagh Wilson; Katie B Freeman; Piers C Emson
Journal:  Brain Res Mol Brain Res       Date:  2003-10-21

8.  Endoplasmic reticulum/golgi nucleotide sugar transporters contribute to the cellular release of UDP-sugar signaling molecules.

Authors:  Juliana I Sesma; Charles R Esther; Silvia M Kreda; Lisa Jones; Wanda O'Neal; Shoko Nishihara; Robert A Nicholas; Eduardo R Lazarowski
Journal:  J Biol Chem       Date:  2009-03-10       Impact factor: 5.157

Review 9.  Transporters of nucleotide sugars, ATP, and nucleotide sulfate in the endoplasmic reticulum and Golgi apparatus.

Authors:  C B Hirschberg; P W Robbins; C Abeijon
Journal:  Annu Rev Biochem       Date:  1998       Impact factor: 23.643

10.  Human immature monocyte-derived dendritic cells express the G protein-coupled receptor GPR105 (KIAA0001, P2Y14) and increase intracellular calcium in response to its agonist, uridine diphosphoglucose.

Authors:  Lisa Skelton; Mike Cooper; Marianne Murphy; Adam Platt
Journal:  J Immunol       Date:  2003-08-15       Impact factor: 5.422
View more
  7 in total

Review 1.  Signalling and pharmacological properties of the P2Y receptor.

Authors:  T K Harden; J I Sesma; I P Fricks; E R Lazarowski
Journal:  Acta Physiol (Oxf)       Date:  2010-03-24       Impact factor: 6.311

Review 2.  UDP-Sugars as Extracellular Signaling Molecules: Cellular and Physiologic Consequences of P2Y14 Receptor Activation.

Authors:  Eduardo R Lazarowski; T Kendall Harden
Journal:  Mol Pharmacol       Date:  2015-03-31       Impact factor: 4.436

3.  Gene-based analysis of regulatory variants identifies 4 putative novel asthma risk genes related to nucleotide synthesis and signaling.

Authors:  Manuel A R Ferreira; Rick Jansen; Gonneke Willemsen; Brenda Penninx; Lisa M Bain; Cristina T Vicente; Joana A Revez; Melanie C Matheson; Jennie Hui; Joyce Y Tung; Svetlana Baltic; Peter Le Souëf; Grant W Montgomery; Nicholas G Martin; Colin F Robertson; Alan James; Philip J Thompson; Dorret I Boomsma; John L Hopper; David A Hinds; Rhiannon B Werder; Simon Phipps
Journal:  J Allergy Clin Immunol       Date:  2016-08-20       Impact factor: 10.793

4.  The UDP-sugar-sensing P2Y(14) receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils.

Authors:  Juliana I Sesma; Silvia M Kreda; Natacha Steinckwich-Besancon; Hong Dang; Rafael García-Mata; T Kendall Harden; Eduardo R Lazarowski
Journal:  Am J Physiol Cell Physiol       Date:  2012-06-06       Impact factor: 4.249

5.  P2Y6 Receptor Activation Promotes Inflammation and Tissue Remodeling in Pulmonary Fibrosis.

Authors:  Tobias Müller; Susanne Fay; Rodolfo Paula Vieira; Harry Karmouty-Quintana; Sanja Cicko; Cemil Korcan Ayata; Gernot Zissel; Torsten Goldmann; Giuseppe Lungarella; Davide Ferrari; Francesco Di Virgilio; Bernard Robaye; Jean-Marie Boeynaems; Eduardo R Lazarowski; Michael R Blackburn; Marco Idzko
Journal:  Front Immunol       Date:  2017-08-22       Impact factor: 7.561

6.  Role of UDP-Sugar Receptor P2Y14 in Murine Osteoblasts.

Authors:  Nicholas Mikolajewicz; Svetlana V Komarova
Journal:  Int J Mol Sci       Date:  2020-04-15       Impact factor: 5.923

7.  UDP-glucose promotes neutrophil recruitment in the lung.

Authors:  Juliana I Sesma; Clarissa D Weitzer; Alessandra Livraghi-Butrico; Hong Dang; Scott Donaldson; Neil E Alexis; Kenneth A Jacobson; T Kendall Harden; Eduardo R Lazarowski
Journal:  Purinergic Signal       Date:  2016-07-15       Impact factor: 3.765
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.