Literature DB >> 19682252

SirA enforces diploidy by inhibiting the replication initiator DnaA during spore formation in Bacillus subtilis.

Jennifer K Wagner1, Kathleen A Marquis, David Z Rudner.   

Abstract

How cells maintain their ploidy is relevant to cellular development and disease. Here, we investigate the mechanism by which the bacterium Bacillus subtilis enforces diploidy as it differentiates into a dormant spore. We demonstrate that a sporulation-induced protein SirA (originally annotated YneE) blocks new rounds of replication by targeting the highly conserved replication initiation factor DnaA. We show that SirA interacts with DnaA and displaces it from the replication origin. As a result, expression of SirA during growth rapidly blocks replication and causes cell death in a DnaA-dependent manner. Finally, cells lacking SirA over-replicate during sporulation. These results support a model in which induction of SirA enforces diploidy by inhibiting replication initiation as B. subtilis cells develop into spores.

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Year:  2009        PMID： 19682252      PMCID： PMC2992877          DOI： 10.1111/j.1365-2958.2009.06825.x

Source DB:  PubMed          Journal:  Mol Microbiol        ISSN： 0950-382X            Impact factor:   3.501


  49 in total

1.  Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli.

Authors:  K Kurokawa; S Nishida; A Emoto; K Sekimizu; T Katayama
Journal:  EMBO J       Date:  1999-12-01       Impact factor: 11.598

2.  Temporal and selective association of multiple sigma factors with RNA polymerase during sporulation in Bacillus subtilis.

Authors:  M Fujita
Journal:  Genes Cells       Date:  2000-02       Impact factor: 1.891

3.  Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.

Authors:  Thierry Doan; Kathleen A Marquis; David Z Rudner
Journal:  Mol Microbiol       Date:  2005-03       Impact factor: 3.501

4.  High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.

Authors:  Masaya Fujita; José Eduardo González-Pastor; Richard Losick
Journal:  J Bacteriol       Date:  2005-02       Impact factor: 3.490

5.  Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A.

Authors:  Masaya Fujita; Richard Losick
Journal:  Genes Dev       Date:  2005-09-15       Impact factor: 11.361

6.  Subcellular positioning of the origin region of the Bacillus subtilis chromosome is independent of sequences within oriC, the site of replication initiation, and the replication initiator DnaA.

Authors:  Melanie B Berkmen; Alan D Grossman
Journal:  Mol Microbiol       Date:  2006-11-27       Impact factor: 3.501

7.  Replication is required for the RecA localization response to DNA damage in Bacillus subtilis.

Authors:  Lyle A Simmons; Alan D Grossman; Graham C Walker
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-17       Impact factor: 11.205

8.  Genome-wide coorientation of replication and transcription reduces adverse effects on replication in Bacillus subtilis.

Authors:  Jue D Wang; Melanie B Berkmen; Alan D Grossman
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-19       Impact factor: 11.205

9.  The forespore line of gene expression in Bacillus subtilis.

Authors:  Stephanie T Wang; Barbara Setlow; Erin M Conlon; Jessica L Lyon; Daisuke Imamura; Tsutomu Sato; Peter Setlow; Richard Losick; Patrick Eichenberger
Journal:  J Mol Biol       Date:  2006-02-08       Impact factor: 5.469

10.  Spo0A, the key transcriptional regulator for entrance into sporulation, is an inhibitor of DNA replication.

Authors:  Virginia Castilla-Llorente; Daniel Muñoz-Espín; Laurentino Villar; Margarita Salas; Wilfried J J Meijer
Journal:  EMBO J       Date:  2006-08-03       Impact factor: 11.598
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  41 in total

1.  RefZ facilitates the switch from medial to polar division during spore formation in Bacillus subtilis.

Authors:  Jennifer K Wagner-Herman; Remi Bernard; Roisin Dunne; Alexandre W Bisson-Filho; Krithika Kumar; Trang Nguyen; Lawrence Mulcahy; John Koullias; Frederico J Gueiros-Filho; David Z Rudner
Journal:  J Bacteriol       Date:  2012-06-22       Impact factor: 3.490

Review 2.  Regulating DnaA complex assembly: it is time to fill the gaps.

Authors:  Alan C Leonard; Julia E Grimwade
Journal:  Curr Opin Microbiol       Date:  2010-10-27       Impact factor: 7.934

Review 3.  Cell Death Pathway That Monitors Spore Morphogenesis.

Authors:  Amanda R Decker; Kumaran S Ramamurthi
Journal:  Trends Microbiol       Date:  2017-04-10       Impact factor: 17.079

4.  Regulation of growth of the mother cell and chromosome replication during sporulation of Bacillus subtilis.

Authors:  Panagiotis Xenopoulos; Patrick J Piggot
Journal:  J Bacteriol       Date:  2011-04-08       Impact factor: 3.490

Review 5.  Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.

Authors:  Tsutomu Katayama; Shogo Ozaki; Kenji Keyamura; Kazuyuki Fujimitsu
Journal:  Nat Rev Microbiol       Date:  2010-03       Impact factor: 60.633

6.  The sporulation protein SirA inhibits the binding of DnaA to the origin of replication by contacting a patch of clustered amino acids.

Authors:  Lilah Rahn-Lee; Houra Merrikh; Alan D Grossman; Richard Losick
Journal:  J Bacteriol       Date:  2011-01-14       Impact factor: 3.490

Review 7.  Random versus Cell Cycle-Regulated Replication Initiation in Bacteria: Insights from Studying Vibrio cholerae Chromosome 2.

Authors:  Revathy Ramachandran; Jyoti Jha; Johan Paulsson; Dhruba Chattoraj
Journal:  Microbiol Mol Biol Rev       Date:  2016-11-30       Impact factor: 11.056

8.  Localization and cellular amounts of the WalRKJ (VicRKX) two-component regulatory system proteins in serotype 2 Streptococcus pneumoniae.

Authors:  Kyle J Wayne; Lok-To Sham; Ho-Ching T Tsui; Alina D Gutu; Skye M Barendt; Susan K Keen; Malcolm E Winkler
Journal:  J Bacteriol       Date:  2010-07-09       Impact factor: 3.490

9.  Bacillus subtilis chromosome organization oscillates between two distinct patterns.

Authors:  Xindan Wang; Paula Montero Llopis; David Z Rudner
Journal:  Proc Natl Acad Sci U S A       Date:  2014-07-28       Impact factor: 11.205

10.  Cryptic protein interactions regulate DNA replication initiation.

Authors:  Lindsay A Matthews; Lyle A Simmons
Journal:  Mol Microbiol       Date:  2018-10-21       Impact factor: 3.501
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