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Literature DB >> 1968171

Rapid detection of herpes-simplex-virus DNA in cerebrospinal fluid of patients with herpes simplex encephalitis.

A H Rowley¹, R J Whitley, F D Lakeman, S M Wolinsky.

Abstract

Herpes-simplex-virus (HSV) DNA in cerebrospinal fluid was amplified by use of the polymerase chain reaction and identified by hybridisation to a specific oligonucleotide probe. Specimens of cerebrospinal fluid (CSF) from 4 of 4 patients with herpes simplex encephalitis were positive for HSV DNA, whereas CSF specimens from 6 patients with other central-nervous-system infections were negative. This technique may expedite diagnosis of herpes simplex encephalitis.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA, Viral

Year: 1990 PMID： 1968171 DOI： 10.1016/0140-6736(90)90667-t

Source DB: PubMed Journal: Lancet ISSN： 0140-6736 Impact factor: 79.321

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Cited

61 in total

Review 1. Molecular diagnosis of herpes simplex virus infections in the central nervous system.

Authors: Y W Tang; P S Mitchell; M J Espy; T F Smith; D H Persing
Journal: J Clin Microbiol Date: 1999-07 Impact factor: 5.948

2. Detection of Herpes simplex virus DNA by real-time PCR.

Authors: H H Kessler; G Mühlbauer; B Rinner; E Stelzl; A Berger; H W Dörr; B Santner; E Marth; H Rabenau
Journal: J Clin Microbiol Date: 2000-07 Impact factor: 5.948

3. Rapid detection of herpes simplex virus DNA in genital ulcers by real-time PCR using SYBR green I dye as the detection signal.

Authors: Carmen Aldea; Carmen P Alvarez; Lola Folgueira; Rafael Delgado; Joaquín R Otero
Journal: J Clin Microbiol Date: 2002-03 Impact factor: 5.948

10. Comparison of specimen processing and nucleic acid extraction by the swab extraction tube system versus the MagNA Pure LC system for laboratory diagnosis of herpes simplex virus infections by LightCycler PCR.

Authors: N C Issa; M J Espy; J R Uhl; W S Harmsen; J N Mandrekar; R E Gullerud; M D Davis; T F Smith
Journal: J Clin Microbiol Date: 2005-03 Impact factor: 5.948

Rapid detection of herpes-simplex-virus DNA in cerebrospinal fluid of patients with herpes simplex encephalitis.

Review 1. Molecular diagnosis of herpes simplex virus infections in the central nervous system.

2. Detection of Herpes simplex virus DNA by real-time PCR.

3. Rapid detection of herpes simplex virus DNA in genital ulcers by real-time PCR using SYBR green I dye as the detection signal.

Review 4. Molecular techniques for clinical diagnostic virology.

5. Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid.

Review 6. Molecular approaches to detecting herpes simplex virus and enteroviruses in the central nervous system.

7. Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by polymerase chain reaction.

8. Spread of herpes simplex virus to the cerebrospinal fluid and the meninges in experimental mouse encephalitis.

9. Involvement of the white matter in the initial stage of herpes simplex encephalitis.

10. Comparison of specimen processing and nucleic acid extraction by the swab extraction tube system versus the MagNA Pure LC system for laboratory diagnosis of herpes simplex virus infections by LightCycler PCR.