| Literature DB >> 19668203 |
Christopher Quince1, Anders Lanzén, Thomas P Curtis, Russell J Davenport, Neil Hall, Ian M Head, L Fiona Read, William T Sloan.
Abstract
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.Mesh:
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Year: 2009 PMID: 19668203 DOI: 10.1038/nmeth.1361
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547