| Literature DB >> 19665978 |
Yohei Shimono1, Maider Zabala, Robert W Cho, Neethan Lobo, Piero Dalerba, Dalong Qian, Maximilian Diehn, Huiping Liu, Sarita P Panula, Eric Chiao, Frederick M Dirbas, George Somlo, Renee A Reijo Pera, Kaiqin Lao, Michael F Clarke.
Abstract
Human breast tumors contain a breast cancer stem cell (BCSC) population with properties reminiscent of normal stem cells. We found 37 microRNAs that were differentially expressed between human BCSCs and nontumorigenic cancer cells. Three clusters, miR-200c-141, miR-200b-200a-429, and miR-183-96-182 were downregulated in human BCSCs, normal human and murine mammary stem/progenitor cells, and embryonal carcinoma cells. Expression of BMI1, a known regulator of stem cell self-renewal, was modulated by miR-200c. miR-200c inhibited the clonal expansion of breast cancer cells and suppressed the growth of embryonal carcinoma cells in vitro. Most importantly, miR-200c strongly suppressed the ability of normal mammary stem cells to form mammary ducts and tumor formation driven by human BCSCs in vivo. The coordinated downregulation of three microRNA clusters and the similar functional regulation of clonal expansion by miR-200c provide a molecular link that connects BCSCs with normal stem cells.Entities:
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Year: 2009 PMID： 19665978 PMCID： PMC2731699 DOI： 10.1016/j.cell.2009.07.011
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582