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Literature DB >> 19652356

Crystallization and preliminary X-ray analysis of a complex formed between the antibiotic simocyclinone D8 and the DNA breakage-reunion domain of Escherichia coli DNA gyrase.

Marcus J Edwards¹, Ruth H Flatman, Lesley A Mitchenall, Clare E M Stevenson, Anthony Maxwell, David M Lawson.

Abstract

Crystals of a complex formed between the 59 kDa N-terminal fragment of the Escherichia coli DNA gyrase A subunit (also known as the breakage-reunion domain) and the antibiotic simocyclinone D8 were grown by vapour diffusion. The complex crystallized with I-centred orthorhombic symmetry and X-ray data were recorded to a resolution of 2.75 A from a single crystal at the synchrotron. DNA gyrase is an essential bacterial enzyme and thus represents an attractive target for drug development.

Entities: Chemical

Mesh：

Substances：

Year: 2009 PMID： 19652356 PMCID： PMC2720350 DOI： 10.1107/S1744309109028097

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

13 in total

1. The CCP4 suite: programs for protein crystallography.

Authors:
Journal: Acta Crystallogr D Biol Crystallogr Date: 1994-09-01

Review 2. Thirty years of Escherichia coli DNA gyrase: from in vivo function to single-molecule mechanism.

Authors: Marcelo Nöllmann; Nancy J Crisona; Paola B Arimondo
Journal: Biochimie Date: 2007-02-24 Impact factor: 4.079

Review 3. DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.

Authors: Allyn J Schoeffler; James M Berger
Journal: Q Rev Biophys Date: 2008-02 Impact factor: 5.318

Review 4. DNA gyrase as a drug target.

Authors: A Maxwell
Journal: Trends Microbiol Date: 1997-03 Impact factor: 17.079

5. Crystal structure of the breakage-reunion domain of DNA gyrase.

Authors: J H Morais Cabral; A P Jackson; C V Smith; N Shikotra; A Maxwell; R C Liddington
Journal: Nature Date: 1997-08-28 Impact factor: 49.962

6. Simocyclinones, novel cytostatic angucyclinone antibiotics produced by Streptomyces antibioticus Tü 6040. I. Taxonomy, fermentation, isolation and biological activities.

Authors: J Schimana; H P Fiedler; I Groth; R Süssmuth; W Beil; M Walker; A Zeeck
Journal: J Antibiot (Tokyo) Date: 2000-08 Impact factor: 2.649

Crystallization and preliminary X-ray analysis of a complex formed between the antibiotic simocyclinone D8 and the DNA breakage-reunion domain of Escherichia coli DNA gyrase.

1. The CCP4 suite: programs for protein crystallography.

Review 2. Thirty years of Escherichia coli DNA gyrase: from in vivo function to single-molecule mechanism.

Review 3. DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.

Review 4. DNA gyrase as a drug target.

5. Crystal structure of the breakage-reunion domain of DNA gyrase.

6. Simocyclinones, novel cytostatic angucyclinone antibiotics produced by Streptomyces antibioticus Tü 6040. I. Taxonomy, fermentation, isolation and biological activities.

7. Simocyclinone D8, an inhibitor of DNA gyrase with a novel mode of action.

Review 8. The ATP-binding site of type II topoisomerases as a target for antibacterial drugs.

Review 9. The integration of macromolecular diffraction data.

Review 10. Scaling and assessment of data quality.

1. Identification of novel bacterial DNA gyrase inhibitors: An in silico study.

Review 2. Structural insights into simocyclinone as an antibiotic, effector ligand and substrate.