BACKGROUND: Terminalia chebula is an ayurvedic drug recommended for the treatment of heart diseases. Earlier studies by the authors validated the beneficial cardioprotective effect of T chebula against isoproterenol-induced myocardial infarction. OBJECTIVES: To evaluate the therapeutic efficacy of T chebula in protecting against isoproterenol-induced lysosomal membrane damage. METHODS: Lysosomal enzyme activities from the serum, heart and lysosomal fractions were determined. The triphenyltetrazolium chloride assay was used to confirm the protective effect of T chebula on the myocardium. RESULTS: Isoproterenol administration produced significant cardiac damage (as seen by the triphenyltetrazolium chloride assay) and significantly altered lysosomal enzyme activities. Pretreatment with an ethanol extract of T chebula was found to retain near normal activities of lysosomal enzymes in rats given T chebula or T chebula plus isoproterenol compared with rats given isoproterenol alone. CONCLUSIONS: Pretreatment with T chebula extract stabilizes the lysosomal membrane and, thus, may have prevented myocardial damage.
BACKGROUND:Terminalia chebula is an ayurvedic drug recommended for the treatment of heart diseases. Earlier studies by the authors validated the beneficial cardioprotective effect of T chebula against isoproterenol-induced myocardial infarction. OBJECTIVES: To evaluate the therapeutic efficacy of T chebula in protecting against isoproterenol-induced lysosomal membrane damage. METHODS: Lysosomal enzyme activities from the serum, heart and lysosomal fractions were determined. The triphenyltetrazolium chloride assay was used to confirm the protective effect of T chebula on the myocardium. RESULTS:Isoproterenol administration produced significant cardiac damage (as seen by the triphenyltetrazolium chloride assay) and significantly altered lysosomal enzyme activities. Pretreatment with an ethanol extract of T chebula was found to retain near normal activities of lysosomal enzymes in rats given T chebula or T chebula plus isoproterenol compared with rats given isoproterenol alone. CONCLUSIONS: Pretreatment with T chebula extract stabilizes the lysosomal membrane and, thus, may have prevented myocardial damage.
Entities:
Keywords:
Isoproterenol; Lysosomes; Myocardial injury; Rats; T chebula
Authors: Emanuella F de Carvalho; André F Nunes; Náiguel C B Silva; João Paulo da Silva Gomes; Renato P de Sousa; Valdelânia G Silva; Paulo H M Nunes; Rosimeire F Santos; Mariana H Chaves; Aldeidia P Oliveira; Rita C M Oliveira Journal: Biol Open Date: 2019-02-27 Impact factor: 2.422